A modified single-prolonged stress model for post-traumatic stress disorder
A modified single-prolonged stress model for post-traumatic stress disorder
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一种改良的创伤后应激障碍单长期应激模型
DOI：
10.1016/j.neulet.2008.06.031
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发表时间：
2008-08-22
影响因子：
2.5
通讯作者：
Tan, Qing Rong
中科院分区：
文献类型：
--
作者：
Wang, Wen;Liu, Yu;Tan, Qing Rong
在大鼠中，单次延长应激(SPS)模型会产生创伤后应激障碍(PTSD)的核心症状，即对创伤线索(条件性恐惧反应)的增强恐惧反应。这种调查工具通常用于创伤后应激障碍的研究。然而，SPS是否会产生创伤后应激障碍的另一种核心症状--过度唤醒(动物模型中的敏感化恐惧反应)，还没有得到评估。同样不清楚的是，在不同的孵化时间后，SP是否能同时增强条件化和敏感化的恐惧反应。在本研究中，大鼠在SPS(SPS&S)手术后立即给予一次性不可避免的足部电击。在不同的孵化时间(1、7或14天)后，通过将应激大鼠重新暴露于电击环境或新环境中的中性音调来测量条件化或敏感化的恐惧反应。此外，选择性5-羟色胺再摄取抑制剂帕罗西汀在SPS和S治疗后14天给予，以测试其对创伤后应激障碍样症状的潜在预防作用。我们观察到条件性恐惧持续存在，并且敏化恐惧随着潜伏期的延长而增加。早期快速干预帕罗西汀可改善创伤后应激障碍样症状的恐惧反应和焦虑行为。我们的数据表明，与其他研究范式相比，这种改进的SPS和S模型可能既新颖又可预测地更好地模拟创伤后应激障碍的临床特征。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
in the rat, single-prolonged stress (SPS) model produces a core symptom of post-traumatic stress disorder (PTSD), the enhanced fear response to the traumatic cue (conditioned fear response). This investigative tool is typically used for PTSD studies. However, whether SPS can produce another core symptom of PTSD, hyperarousal (the sensitized fear response in animal models), has not been evaluated. It is also not clear whether SPS can enhance both conditioned and sensitized fear responses after different incubation times. In this study, a single inescapable electric foot shock was given to rats immediately after SPS procedures (SPS&S). After different incubation times (1, 7 or 14 days), the conditioned or sensitized fear response was measured by re-exposing the stressed rats to the shock context or a neutral tone in a novel environment. Additionally, paroxetine, a selective serotonin reuptake inhibitor (SSRI) was administered after SPS&S for 14 days to test its potential preventive effect on PTSD-like symptoms. We observed that conditioned fear persisted and sensitized fear increased with ongoing incubation times after SPS&S. Early rapid intervention with paroxetine after SPS&S ameliorated PTSD-like symptoms in both fear responses and anxiety behaviors. Our data suggests that this modified SPS&S model may be both novel and predictably mimic the clinical characteristics of PTSD better than other investigative paradigms. (C) 2008 Elsevier Ireland Ltd. All rights reserved.