Reduced cell attachment to poly(2-hydroxyethyl methacrylate)-coated ventricular catheters in vitro
Reduced cell attachment to poly(2-hydroxyethyl methacrylate)-coated ventricular catheters in vitro
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DOI：
10.1002/jbm.b.33915
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发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Shain, William
中科院分区：
文献类型：
--
作者：
Hanak, Brian W.;Hsieh, Chia-Yun;Shain, William
大多数脑积水患者依赖脑室腹腔分流术转移多余的脑脊液。不幸的是，这些分流是容易失败的，并且超过一半的危及生命的儿科失败是由大脑的常驻免疫细胞、反应性小胶质细胞和星形胶质细胞阻塞脑室导管引起的。聚（甲基丙烯酸2-羟乙酯）（PHEMA）水凝胶广泛用于生物医学植入物。PHEMA的极端亲水性赋予了对蛋白质污染的抗性，使其成为脑室导管的强有力的候选涂层。随着引发化学气相沉积（iCVD）的出现，一种通过将气态反应物引入真空反应器中在基底表面上产生薄膜形式的聚合物的无溶剂涂覆技术，现在可以在复杂的三维基底表面上施加均匀的聚合物涂层。iCVD用于用PHEMA涂覆市售脑室导管。使用傅里叶变换红外光谱和X射线光电子能谱确认导管表面的化学结构。用扫描电子显微镜对PHEMA涂层形貌进行了表征。在含有共培养的星形胶质细胞和小胶质细胞的体外脑积水导管生物反应器中，将PHEMA涂层导管与未涂层临床级导管进行对比试验，结果显示在17天和6周时间点时，PHEMA涂层导管的细胞附着显著减少。(c)2017 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，106 B：1268-1279，2018。
The majority of patients with hydrocephalus are dependent on ventriculoperitoneal shunts for diversion of excess cerebrospinal fluid. Unfortunately, these shunts are failure-prone and over half of all life-threatening pediatric failures are caused by obstruction of the ventricular catheter by the brain's resident immune cells, reactive microglia and astrocytes. Poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogels are widely used for biomedical implants. The extreme hydrophilicity of PHEMA confers resistance to protein fouling, making it a strong candidate coating for ventricular catheters. With the advent of initiated chemical vapor deposition (iCVD), a solvent-free coating technology that creates a polymer in thin film form on a substrate surface by introducing gaseous reactant species into a vacuum reactor, it is now possible to apply uniform polymer coatings on complex three-dimensional substrate surfaces. iCVD was utilized to coat commercially available ventricular catheters with PHEMA. The chemical structure was confirmed on catheter surfaces using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. PHEMA coating morphology was characterized by scanning electron microscopy. Testing PHEMA-coated catheters against uncoated clinical-grade catheters in an in vitro hydrocephalus catheter bioreactor containing co-cultured astrocytes and microglia revealed significant reductions in cell attachment to PHEMA-coated catheters at both 17-day and 6-week time points. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1268-1279, 2018.