Effects of myoinhibitory peptides on food intake in the German cockroach
Effects of myoinhibitory peptides on food intake in the German cockroach
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DOI：
10.1111/j.1365-3032.2006.00513.x
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发表时间：
2006-09-01
影响因子：
1.5
通讯作者：
Belles, Xavier
中科院分区：
文献类型：
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作者：
Aguilar, Ruth;Maestro, Jose L.;Belles, Xavier
昆虫肌抑肽是通过抑制内脏肌肉收缩而发现的。本研究测定了三种甘丙素相关肌动抑制多肽(Mas-MIP II：GWQDLNSAW-NH2、GRB-AST-B1：GWQDLNGGW-NH2和GRB-AST-B3：AWRDLSGGW-NH2)对德国小蠊成虫肠道肌力的影响。(双翅目，斑潜蝇科)。这三种多肽对前肠和后肠收缩都有很强的抑制作用，所有病例的ID50值都在纳摩尔范围内。此外，还研究了这三种多肽对饥饿雌性蟑螂摄食量的调节作用。结果表明，GRB-AST-B3是最有活性的多肽，在15-50 mg剂量下抑制摄食量60%-80%，其次是GRB-AST-B1(在50 mg剂量下抑制45%的食物摄入)，而Mas-MIP II即使在50 mg剂量下也没有活性。这三种多肽之间的差异可能是由于它们的结构对活性的不同影响或由于不同的降解。这些结果表明，体外肠肌调节活动和拒食效应并不总是相关的。
Insect myoinhibitory peptides were discovered through their inhibitory activity on visceral muscle contraction. The present study tests the antimyotropic gut properties of three galanin-related myoinhibitory peptides (Mas-MIP II: GWQDLNSAW-NH2; Grb-AST-B1: GWQDLNGGW-NH2; and Grb-AST-B3: AWRDLSGGW-NH2) in adult females of the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae). The three peptides elicit a strong inhibitory effect on both foregut and hindgut contractions, with ID50 values in all the cases within the nanomolar range. In addition, the modulatory effects of these three peptides on food intake are studied on previously starved female cockroaches. The results show that Grb-AST-B3 is the most active peptide, inhibiting food intake by 60-80% at doses between 15 and 50 mg, followed by Grb-AST-B1 (45% inhibition of food intake at the 50 mg dose), whereas Mas-MIP II is inactive even at the 50 mg dose. The differences between the three peptides may be due to a differential effect of their structure on activity or to a differential degradation. These results show that myomodulatory gut activity in vitro and antifeeding effects do not always correlate.