Structural characterisation of human recombinant glycohormones follitropin, lutropin and choriogonadotropin expressed in Chinese hamster ovary cells
Structural characterisation of human recombinant glycohormones follitropin, lutropin and choriogonadotropin expressed in Chinese hamster ovary cells
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DOI：
10.1111/j.1432-1033.1996.0608r.x
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发表时间：
1996-12-15
期刊：
影响因子：
--
通讯作者：
Pucci, P
中科院分区：
文献类型：
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作者：
Amoresano, A;Siciliano, R;Pucci, P
在CHO细胞中表达的人重组促性腺激素卵泡激素、尿路激素和绒毛膜促性腺激素的α链和β链在蛋白质和碳水化合物水平上进行了结构表征。这三条α链具有相同的氨基酸序列，但它们表现出不同的糖基化模式。检测到的低聚糖结构属于不同程度唾液酰化的络合型聚糖。部分蛋白水解过程发生在卵泡蛋白链的n端和lutropin链的c端。三个β链上的n链聚糖被发现含有聚焦化和唾液化的二链和三链络合物型结构。卵泡素β链显示在触角上存在n -乙酰乳胺重复序列。重组糖激素的整体结构与它们的天然对应物相对应，除了缺少硫酸末端糖基化。
The alpha and beta chains from human recombinant gonadotropins follitropin, lutropin and choriogonadotropin expressed in CHO cells have been structurally characterised both at the protein and at the carbohydrate level by using advanced mass spectrometric procedures. The three alpha chains share the same amino acid sequence while they display different glycosylation patterns. The oligosaccharide structures detected belong to the complex-type glycans with different degree of sialylation. Partial proteolytic processing occurred at the N-terminus of the follitropin beta chain and at the C-terminus of the lutropin beta chain. The N-linked glycans from the three beta chains were found to contain fucosylated and sialylated diantennary and triantennary complex-type structures. The follitropin beta chain showed the presence of N-acetyllactosamine repeats on the antennae.The overall structure of the recombinant glycohormones corresponds to their natural counterparts with the exception that sulphated terminal glycosylation is missing.