Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate
Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate
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DOI：
10.3382/ps/pew158
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发表时间：
2016-10-01
期刊：
影响因子：
4.4
通讯作者：
Lee, J. H.
中科院分区：
文献类型：
--
作者：
Kamble, N. M.;Jawale, C. V.;Lee, J. H.
基于细菌幽灵的疫苗开发已应用于多种革兰氏阴性细菌。开发的肠炎沙门氏菌（S. Enteritidis）幽灵因其免疫原性和增强的生物安全潜力而成为有前途的候选疫苗。在本研究中，我们旨在评估肠炎沙门氏菌幽灵疫苗对体外鸡骨髓源性树突状细胞（chBM-DC）成熟的免疫刺激作用。用肠炎沙门氏菌幽灵疫苗候选物刺激未成熟的 chBM-DC。 CD40、CD80 和 MHC-II 分子表达上调表明该疫苗有效刺激了 chBM-DC 的成熟事件。未成熟的 BM-DC 通过增加 IL-6 和 IL-12p40 细胞因子的表达来响应肠炎沙门氏菌菌影的刺激。此外，肠炎沙门氏菌幽灵刺激的 chBM-DC 诱导共培养的自体 CD4+ T 细胞中 IFN-γ 和 IL-2 的显着表达。总之，我们的数据表明候选肠炎沙门氏菌幽灵疫苗能够激活 chBM-DC 并与之相互作用。目前的研究结果可能有助于合理设计基于沙门氏菌鬼的树突状细胞异源抗原递送平台。
Bacterial Ghost-based vaccine development has been applied to a variety of gram-negative bacteria. Developed Salmonella Enteritidis (S. Enteritidis) ghost are promising vaccine candidates because of their immunogenic and enhanced biosafety potential. In this study, we aimed to evaluate the immunostimulatory effect of a S. Enteritidis ghost vaccine on the maturation of chicken bone marrow-derived dendritic cells (chBM-DCs) in vitro. The immature chBM-DCs were stimulated with S. Enteritidis ghost vaccine candidate. The vaccine efficiently stimulated maturation events in chBM-DCs, indicated by up-regulated expression of CD40, CD80, and MHC-II molecules. Immature BM-DCs responded to stimulation with S. Enteritidis ghost by increased expression of IL-6 and IL-12p40 cytokines. Also, S. Enteritidis ghost stimulated chBM-DCs induced the significant expression of IFN-gamma and IL-2 in co-cultured autologous CD4+ T cells. In conclusion, our data suggest that S. Enteritidis ghost vaccine candidate is capable of activating and interacting with chBM-DCs. The results from current study may help for rational designing of Salmonella ghost based heterologous antigen delivery platforms to dendritic cells.