Laccase-catalyzed polymerization of tyrosine-containing peptides
Laccase-catalyzed polymerization of tyrosine-containing peptides
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DOI：
10.1111/j.1742-4658.2005.04786.x
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发表时间：
2005-07-01
期刊：
影响因子：
5.4
通讯作者：
Steffensen, CL
中科院分区：
文献类型：
--
作者：
Mattinen, ML;Kruus, K;Steffensen, CL
研究了在阿魏酸(FA)存在和不存在的情况下，漆酶催化酪氨酸和含酪氨酸多肽的聚合反应。采用先进的光谱手段，如MALDI-TOF MS、EPR、FTIR显微镜和HPLC-荧光，以及更传统的分析工具：氧耗测量和SDS/PAGE对反应机理进行了研究。漆酶被发现氧化酪氨酸和含酪氨酸的多肽，从而使化合物聚合。化合物之间的共价键是以醚键形式存在的。在聚合物中只检测到少量的二氨基偶氮键。当将FA加入反应混合物中时，发现它已融入到聚合物结构中。因此，除了均聚物外，在反应中还形成了含有两个或四个FA残基的不同杂化聚合物。
Laccase-catalyzed polymerization of tyrosine and tyrosine-containing peptides was studied in the presence and absence of ferulic acid (FA). Advanced spectroscopic methods such as MALDI-TOF MS, EPR, FTIR microscopy and HPLC-fluorescence, as well as more conventional analytical tools: oxygen consumption measurements and SDS/PAGE were used in the reaction mechanism studies. Laccase was found to oxidize tyrosine and tyrosine-containing peptides, with consequent polymerization of the compounds. The covalent linkage connecting the compounds was found to be an ether bond. Only small amounts of dityrosine bonds were detected in the polymers. When FA was added to the reaction mixtures, it was found to be incorporated into the polymer structure. Thus, in addition to homopolymers, different heteropolymers containing two or four FA residues were formed in the reactions.