Microarray Identification of Clostridium difficile Core Components and Divergent Regions Associated with Host Origin
Microarray Identification of Clostridium difficile Core Components and Divergent Regions Associated with Host Origin
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DOI：
10.1128/jb.00222-09
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发表时间：
2009-06-15
影响因子：
3.2
通讯作者：
Chang, Yung-Fu
中科院分区：
文献类型：
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作者：
Janvilisri, Tavan;Scaria, Joy;Chang, Yung-Fu
艰难梭菌是一种革兰氏阳性、形成孢子的肠道厌氧菌，可感染人类和多种动物物种。近年来，人类C.艰难梭菌感染明显增加本研究对73 C.艰难梭菌菌株分离自人类，马，牛和猪，通过比较基因组杂交与微阵列含有编码序列的C。艰难梭菌菌株630和QCD-32 g58。对C.艰难梭菌菌株630用作参考以定义C. difficile，并探讨宿主起源和遗传多样性之间的相关性。菌株630中约16%的基因在所有菌株中高度保守，代表了C.很难供试菌株中的缺失或分歧基因分布在整个C. difficile 630基因组和所有预测的功能类别。有趣的是，某些基因在来自特定宿主物种的菌株中是保守的，但在具有其他宿主来源的菌株中是不同的。这些信息提供了深入了解基因组的变化，可能有助于主机的适应。由于C.艰难梭菌菌株，从这项研究的核心基因列表提供了第一步的诊断芯片的建设。很难
Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from humans, horses, cattle, and pigs by comparative genomic hybridization with microarrays containing coding sequences from C. difficile strains 630 and QCD-32g58. The sequenced genome of C. difficile strain 630 was used as a reference to define a candidate core genome of C. difficile and to explore correlations between host origins and genetic diversity. Approximately 16% of the genes in strain 630 were highly conserved among all strains, representing the core complement of functional genes defining C. difficile. Absent or divergent genes in the tested strains were distributed across the entire C. difficile 630 genome and across all the predicted functional categories. Interestingly, certain genes were conserved among strains from a specific host species, but divergent in isolates with other host origins. This information provides insight into the genomic changes which might contribute to host adaptation. Due to a high degree of divergence among C. difficile strains, a core gene list from this study offers the first step toward the construction of diagnostic arrays for C. difficile.