Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato.
Identification of genes regulated by a jasmonate- and salt-inducible transcription factor JRE3 in tomato.
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DOI：
10.5511/plantbiotechnology.19.0206a
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发表时间：
2019-03
影响因子：
1.6
通讯作者：
A. Abdelkareem;Chonprakun Thagun;S. Imanishi;T. Hashimoto;T. Shoji
中科院分区：
文献类型：
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作者：
A. Abdelkareem;Chonprakun Thagun;S. Imanishi;T. Hashimoto;T. Shoji
番茄中，APETALA2/ERF(AP2/ERF)家族的转录因子茉莉酸应答ERF3(JRE3)是类固醇糖生物碱(SGA)生物合成的主要转录调控因子JRE4的同源基因。在番茄基因组中，JRE3与JRE4及相关的JRE1、JRE2和JRE5处于一个基因簇中，而JRE6则以单个体形式存在于不同的染色体上。除JRE4和JRE6外，所有的JRE都被茉莉酸(JAs)诱导，而氯化钠(NaC L)处理显著增加了JRE的表达。在本研究中，为了深入了解茉莉酸和氯化钠诱导的JRE3的调控功能，本研究利用基因芯片技术在转基因番茄毛状根中鉴定了β-雌二醇诱导的JRE3过表达的一系列基因。目前还没有发现参与SGA途径的基因，证实了JRE3和JRE4在功能上的差异。在JRE3调控的基因中，我们研究了胁迫诱导的苹果酸合成酶和液泡膜二羧酸转运蛋白基因的表达，这两个基因都参与了苹果酸的积累。在瞬时反式激活分析中，我们发现JRE4的两个末端区域，而不是一个中心DNA结合域，对于JRE4调节子相关基因的诱导是必不可少的。对JRE的功能分化进行了讨论。
In Solanum lycoperisicum (tomato), a transcription factor of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family, JASMONATE-RESPONSIVE ERF 3 (JRE3), is a closest homolog of JRE4, a master transcriptional regulator of steroidal glycoalkaloid (SGA) biosynthesis. In tomato genome, JRE3 resides in a gene cluster with JRE4 and related JRE1, JRE2, and JRE5, while JRE6 exists as a singleton on a different chromosome. All of the JREs are induced by jasmonates (JAs), whereas sodium chloride (NaCl) treatment drastically increases the expression of the JREs except for JRE4 and JRE6. In this study, to get insights into the regulatory function of the JA- and NaCl-inducible JRE3, a series of genes upregulated by β-estradiol-induced overexpression of JRE3 are identified with microarray analysis in transgenic tomato hairy roots. No gene involved in the SGA pathway has been identified through the screening, confirming the functional distinction between JRE3 and JRE4. Among the JRE3-regulated genes, we characterize the stress-induced expression of genes encoding malate synthase and tonoplast dicarboxylate transporter both involved in malate accumulation. In transient transactivation assay, we reveal that both terminal regions of JRE4, but not a central DNA-binding domain, are indispensable for the induction of a gene involved in the JRE4 regulon. Functional differentiation of the JREs is discussed.