Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering.
Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering.
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DOI：
10.1021/acs.biomac.6b00684
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发表时间：
2016-10-10
影响因子：
6.2
通讯作者：
Liu JC
中科院分区：
文献类型：
--
作者：
Vázquez-Portalatı N N;Kilmer CE;Panitch A;Liu JC
提供天然细胞外基质中存在的信号的生物材料已被提议作为支持改善软骨再生的支架。本研究通过表征由I型和II型胶原蛋白混合物制成的凝胶，利用II型胶原蛋白的生物活性和I型胶原蛋白的上级机械性能。胶原蛋白共混物水凝胶能够掺入两种类型的胶原蛋白和保留的硫酸软骨素和透明质酸。冷冻扫描电子显微镜图像显示，3：1比例的I型胶原与II型胶原凝胶的空隙率（36.4%）低于1：1凝胶（46.5%）。与1：1凝胶（G *=1.2 Pa）相比，3：1凝胶（G *=5.0 Pa）的复数模量更大。与其他共混物相比，3：1共混物一致地形成具有上级机械性能的凝胶，并且具有作为关节软骨工程支架实施的潜力。
Biomaterials that provide signals present in the native extracellular matrix have been proposed as scaffolds to support improved cartilage regeneration. This study harnesses the biological activity of collagen type II and the superior mechanical properties of collagen type I by characterizing gels made of collagen type I and II blends. The collagen blend hydrogels were able to incorporate both types of collagen and retained chondroitin sulfate and hyaluronic acid. Cryo-scanning electron microscopy images showed that the 3:1 ratio of collagen type I to type II gels had a lower void space percentage (36.4%) than the 1:1 gels (46.5%). The complex modulus was larger for the 3:1 gels (G*=5.0 Pa) compared to the 1:1 gels (G*=1.2 Pa). The 3:1 blend consistently formed gels with superior mechanical properties compared to the other blends and has the potential to be implemented as a scaffold for articular cartilage engineering.