Position-specific expression of Hox genes along the gastrointestinal tract
Position-specific expression of Hox genes along the gastrointestinal tract
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DOI：
10.1111/j.1741-4520.2003.00004.x
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发表时间：
2004-03-01
影响因子：
1.3
通讯作者：
Kosaki, Kenjiro
中科院分区：
文献类型：
--
作者：
Yahagi, Naohisa;Kosaki, Rika;Kosaki, Kenjiro
Hox基因在早期胚胎沿着前后轴的形态发生中起关键作用。在哺乳动物中，具有广泛同源性的39个Hox基因被组织成13个旁系同源组，在4条不同的染色体上形成4个簇。每个簇内的基因以3'至5'方向排列，并在椎骨、四肢和内脏（包括胃肠道）中沿着前后轴以时间和空间协调的方式沿着表达，但对其空间表达知之甚少。成人胃肠道。应用SYBR绿色（TM）荧光定量PCR方法，对人Hox基因在成人胃肠道组织中的表达进行了定量分析，包括食管、胃、十二指肠、空肠、回肠、回盲肠、盲肠、升结肠、横结肠、降结肠和直肠。将Hox基因表达标准化为甘油醛-3-磷酸脱氢酶（GAPDH）基因表达。用多变量方法分析空间表达模式。所有39个Hox基因的表达水平可以以可重复的方式测量。在前肠来源的片段中具有较高表达的基因在后肠来源的片段中倾向于具有较低表达，而在前肠来源的片段中具有较低表达的基因在后肠来源的片段中倾向于具有较高表达。主成分分析和permax分析揭示了Hox基因沿着成人胃肠道前后轴的位置特异性表达模式。该模式概括了胚胎胃肠道中的表达模式。我们认为Hox基因可能在肠上皮细胞的位置特异性再生过程中发挥关键作用。
Hox genes play a critical role in morphogenesis of the early embryo along the anteroposterior axis. In mammals, 39 Hox genes with extensive homology are organized into 13 paralogous groups, forming four clusters on four separate chromosomes. The genes within each cluster are arranged in a 3' to 5' direction and expressed in a temporally and spatially coordinated manner along the anteroposterior axis in the vertebrae, limbs and viscera, including the gastrointestinal tract, but little is known about their spatial expression in the adult gastrointestinal tract. We used the quantitative polymerase chain reaction (PCR) intercalater method with SYBR Green(TM) to quantify human Hox gene expression in the adult gastrointestinal tract tissue: esophagus, stomach, duodenum, jejunum, ileum, ileocecum, cecum, ascending colon, transverse colon, descending colon and rectum. Hox gene expression was normalized to glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) gene expression. The spatial expression pattern was analyzed by the multivariate method. The expression level of all 39 Hox genes could be measured in a reproducible manner. Genes with higher expression in the foregut-derived segments tended to have lower expression in hindgut-derived segments, whereas those with low expression in the former tended to have higher in the latter. Principal components analysis and permax analysis revealed a position-specific expression pattern of Hox genes along the anteroposterior axis of the adult gastrointestinal tract. The pattern recapitulates the expression pattern in the embryonic gastrointestinal tract. We suggest that Hox genes may play a pivotal role in the position-specific regenerative process of intestinal epithelial cells.