Osteogenic differentiation of BMSCs on MoS2 composite nanofibers with different cell seeding densities
Osteogenic differentiation of BMSCs on MoS2 composite nanofibers with different cell seeding densities
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不同细胞接种密度的MoS2复合纳米纤维上BMSCs的成骨分化
DOI：
10.1007/s13204-020-01473-0
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发表时间：
2020-06-04
影响因子：
--
通讯作者：
Li, Yan
中科院分区：
文献类型：
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作者：
Luo, Shulu;Wu, Shuyi;Li, Yan
二硫化钼（MoS2）具有独特的性能，可用于各种生物医学应用。由于其独特的特性和促进成骨分化的作用，该材料被广泛研究。然而，细胞密度对基于二硫化钼的生物材料和细胞之间成骨分化的影响尚不清楚。本研究以MoS2/聚丙烯腈（PAN）复合纳米纤维为底物，研究细胞密度对骨髓间充质干细胞（BMSCs）成骨分化的影响。我们建立不同的实验组，增加细胞播种密度，研究细胞行为、生物相容性、增殖和成骨特性。结果表明，二硫化钼/聚丙烯腈复合纳米纤维对成骨分化具有正向调节作用。更重要的是，在标准培养条件下，1.0 × 104个细胞/cm2是BMSCs成骨分化最有效、最合适的细胞播种密度。我们的研究结果表明，当BMSCs在基于二硫化钼的生物材料上培养时，最佳的成骨细胞密度对于BMSCs的成骨分化至关重要。
Molybdenum disulphide (MoS2) exhibits unique properties that are useful for various biomedical applications. Owing to its distinct characteristics and osteogenic differentiation promotion effect, this material has been studied extensively. However, the effect of cell density on osteogenic differentiation between MoS2-based biomaterials and cells is still unknown. In this study, we used MoS2/polyacrylonitrile (PAN) composite nanofibres as substrates to evaluate the effect of cellular density on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). We created different experimental groups with increasing cell seeding densities and investigated cellular behaviours, biocompatibility, proliferation and osteogenic properties. The results show that MoS2/PAN composite nanofibres can positively regulate osteogenic differentiation. More importantly, in the presence of standard culture conditions, 1.0 × 104 cells/cm2 is the most efficient and suitable cell seeding density for BMSCs osteogenic differentiation. Our findings suggest that the optimal cell density for osteogenesis is vital for the osteogenic differentiation of BMSCs when these cells are cultured onto MoS2-based biomaterials.