Ketamine suppresses intestinal TLR4 expression and NF-kappaB activity in lipopolysaccharide-treated rats.
Ketamine suppresses intestinal TLR4 expression and NF-kappaB activity in lipopolysaccharide-treated rats.
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发表时间：
2006-12
影响因子：
1.9
通讯作者：
Min Yu;Dan-bing Shao;Jianjun Yang;S. Feng;Jianguo Xu
中科院分区：
文献类型：
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作者：
Min Yu;Dan-bing Shao;Jianjun Yang;S. Feng;Jianguo Xu
目的观察氯胺酮对脂多糖(LPS)诱导的大鼠肠道Toll样受体4(TLR4)表达和核因子-kappaB(NF-kappaB)活性的影响。方法6组大鼠分为生理盐水对照组、内毒素(5 mg/kg)+生理盐水组、内毒素(5 mg/kg)+氯胺酮(0.5 mg/kg)组、内毒素(5 mg/kg)+氯胺酮(2.5 mg/kg)组、内毒素(5 mg/kg)+氯胺酮(10 mg/kg)组、氯胺酮(10 mg/kg)组。用逆转录聚合酶链式反应(RT-PCR)检测肠组织TLR4mRNA的表达，用凝胶迁移率改变法(EMSA)检测内毒素注射后1h、3h、5h肠组织中核因子-kappaB的活性。结果内毒素可增加大鼠肠道TLR4的表达和核因子-kappaB的活性。0.5、2.5和10 mg/kg的氯胺酮可抑制内毒素诱导的TLR4表达和核因子-kappaB活性的升高。单靠氯胺酮是没有效果的。结论氯胺酮可能通过抑制TLR4的表达而抑制内毒素诱导的大鼠肠组织中核因子-kappaB的活化。
AIM To investigate whether ketamine suppresses lipopolysaccharide (LPS)-induced increase in Toll-like receptor 4 (TLR4) expression and nuclear factor-kappa B (NF-kappaB) activity in the intestines of rats. METHODS Six groups of rats received one of the following: normal saline control, LPS (5 mg/kg) plus saline, LPS (5 mg/kg) plus ketamine (0.5 mg/kg), LPS (5 mg/kg) plus ketamine (2.5 mg/kg), LPS (5 mg/kg) plus ketamine (10 mg/kg), or ketamine (10 mg/kg) alone. Intestinal TLR4 mRNA expression was analyzed by reverse transcription polymerase chain reaction (RT-PCR), and NF-kappaB activity was tested by electrophoretic mobility shift assay (EMSA) 1, 3, or 5 hours after the LPS injection. RESULTS Lipopolysaccharide increased TLR4 expression and NF-kappaB activity in the intestines of rats. Ketamine at the dosage of 0.5, 2.5, and 10 mg/kg suppressed the LPS-induced increase in TLR4 expression and NF-kappaB activity. Ketamine alone had no effect. CONCLUSION The study demonstrated that ketamine inhibits NF-kappaB activation in the intestines of LPS-treated rats, possibly by suppressing TLR4 expression.