Inactivation of Efflux Pumps Abolishes Bacterial Biofilm Formation
Inactivation of Efflux Pumps Abolishes Bacterial Biofilm Formation
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DOI：
10.1128/aem.01310-08
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Klemm, Per
中科院分区：
文献类型：
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作者：
Kvist, Malin;Hancock, Viktoria;Klemm, Per
细菌生物膜在医疗保健和工业领域造成许多问题；值得注意的是，生物膜与大量感染有关。生物膜中的细菌对抗生素特别有抵抗力，因此很难根除与生物膜相关的感染。细菌依靠外排泵来清除有毒物质。我们发现外排泵在细菌生物膜中高度活跃，因此使外排泵成为抗生物膜措施的有吸引力的目标。许多外排泵抑制剂(EPI)是已知的。 EPI 被证明可以减少生物膜的形成，并且它们结合起来可以完全消除生物膜的形成。此外，EPIs 能够阻断生物膜的抗生素耐受性。这项可行性研究的结果可能为生物膜相关感染的新治疗方法铺平道路，并可用于一般生物膜的预防。
Bacterial biofilms cause numerous problems in health care and industry; notably, biofilms are associated with a large number of infections. Biofilm-dwelling bacteria are particularly resistant to antibiotics, making it hard to eradicate biofilm-associated infections. Bacteria rely on efflux pumps to get rid of toxic substances. We discovered that efflux pumps are highly active in bacterial biofilms, thus making efflux pumps attractive targets for antibiofilm measures. A number of efflux pump inhibitors (EPIs) are known. EPIs were shown to reduce biofilm formation, and in combination they could abolish biofilm formation completely. Also, EPIs were able to block the antibiotic tolerance of biofilms. The results of this feasibility study might pave the way for new treatments for biofilm-related infections and may be exploited for prevention of biofilms in general.