Increase of poorly proliferated p63(+)/Ki67(+) basal cells forming multiple layers in the aberrant remodeled epithelium in nasal polyps
Increase of poorly proliferated p63(+)/Ki67(+) basal cells forming multiple layers in the aberrant remodeled epithelium in nasal polyps
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鼻息肉异常重塑上皮中增殖不良的 p63( )/Ki67( ) 基底细胞增加，形成多层
作者：
Zhao L.;Li Y. Y.;Li C. W.;Chao S. S.;Liu J.;Nam H. N.;Dung N. T. N.;Shi L.;Wang D. Y.
背景：p63（基底细胞标记物）异位表达导致的异常上皮重塑是慢性炎症气道上皮（例如鼻息肉（NP））中常见的重要病理现象。 ..方法：对 55 名 NP 患者和 18 名健康对照（下鼻甲）进行活检。在 NP 患者中，15 例接受了口服和鼻腔类固醇治疗，因此在治疗前后进行了两组 NP 活检。在这些样本中研究了 p63、Ki67、IV 型 β-微管蛋白和细胞周期标记物。 ..结果：NP 的增生上皮（1.53 倍，P < 0.0001）和鳞状化生上皮（2.02 倍，P < 0.0001）中 p63（+）细胞的数量显着高于健康对照。增殖性基底细胞分为三种类型（p63(+)/Ki67(+)），它们处于细胞周期的不同阶段，例如G1期（I型细胞）、S至G2期（II型细胞）和有丝分裂（III型细胞）。重要的是，一些 I 型细胞可能在增殖后停滞，尽管它们可能仍然是 p63(+)/Ki67(+)。在健康的上皮中，I型和II型细胞的比例几乎为50：50。然而，在增生性上皮（34.85％，P = 0.012）和鳞状化生上皮（30.77％，P = 0.02）中发现较少的II型细胞，同时存在III型细胞（3.45％，P = 0.01）。这些发现在类固醇治疗后没有改变。 ..结论：增殖不良的基底细胞形成多层的增加，可能对基底细胞标记物进行染色，但不会形成适当的表皮屏障，是 NP 异常重塑上皮的重要组织病理学现象。
Background: Aberrant epithelial remodeling with the ectopic expression of p63 ( basal cell markers) is an important pathologic phenomenon seen in chronically inflamed airway epithelium such as in nasal polyps (NPs). ..Methods: Biopsies were obtained from 55 NP patients and 18 healthy controls ( inferior turbinate). Among NP patients, 15 were treated with oral and nasal steroids, so that two sets of NP biopsies were taken before and after the treatments. p63, Ki67, type IV beta-tubulin, and cell cycle markers were investigated in these specimens. ..Results: The number of p63(+) cells is significantly higher in both hyperplastic (1.53-fold, P < 0.0001) and squamous metaplastic (2.02- fold, P < 0.0001) epithelium from NPs than from healthy controls. There are three types of proliferative basal cells (p63(+)/Ki67(+)) which are in different phases of the cell cycle, such as G1 phase (type I cells), S to G2 phase (type II cells), and mitosis (type III cells). Of importance, some type I cells may arrest after proliferation although they may still be p63(+)/Ki67(+). In healthy epithelium, the ratio of the type I and II cells is almost 50: 50. However, less type II cells are found in hyperplastic epithelium (34.85%, P = 0.012) and in squamous metaplastic epithelium (30.77%, P = 0.02) together with the presence of type III cells (3.45%, P = 0.01). These findings were not changed after steroid treatments. ..Conclusions: An increase of poorly proliferated basal cells forming multiple layers, which may stain for basal cell markers but does not form a proper epidermal barrier, is an important histopathologic phenomenon in aberrant remodeled epithelium of NPs.