Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure.
Effects of the activated mitogen-activated protein kinase pathway via the c-ros receptor tyrosine kinase on the T47D breast cancer cell line following alcohol exposure.
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DOI：
10.3892/or.2012.2209
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发表时间：
2013-03
期刊：
影响因子：
4.2
通讯作者：
Chai YG
中科院分区：
文献类型：
--
作者：
Lee HT;Kim SK;Choi MR;Park JH;Jung KH;Chai YG
与影响女性的其他癌症相比，乳腺癌与饮酒密切相关。然而，酒精诱发的乳腺癌的致癌原理和相关的转移机制尚未确定。为了观察酒精对乳腺癌细胞生长调控的影响，我们用凝胶蛋白质组学方法对酒精暴露的人乳腺癌T47D细胞的差异表达蛋白质进行了鉴定。C-ros受体酪氨酸激酶(ROS1)的表达增加，并被自身磷酸化激活，从而通过丝裂原激活的蛋白激酶(MAPK)途径激活丝裂原和应激激活的蛋白激酶1(MSK1)，进而激活MSK1，进而激活细胞核中的组蛋白3丝氨酸10(H3S10p)残基。H3S10磷酸化水平的增加导致c-fos等即刻早期基因的表达水平升高。这项研究表明，当乳腺癌细胞暴露在酒精中时，磷酸化的ROS1通过MAPK通路中的ERK1/2激活MSK1，然后诱导组蛋白残基的修饰，通过14-3-3蛋白的募集来调节基因的表达，导致乳腺癌细胞的增殖缺乏控制。
Compared to other cancers affecting women, breast cancer is significantly associated with alcohol consumption. However, the principles underlying the carcinogenesis of alcohol-induced breast cancer and the related metastatic mechanisms have yet to be established. To observe the effect of alcohol on the growth regulation in breast cancer cells, we identified differentially expressed proteins in alcohol-exposed human breast cancer T47D cells using gel-based proteomics analysis. The expression of c-ros receptor tyrosine kinase (ROS1) was increased and activated by autophosphorylation, thereby activating mitogen- and stress-activated protein kinase 1 (MSK1) through the mitogen-activated protein kinase (MAPK) pathway; activated MSK1, in turn, phosphorylated histone 3 serine 10 (H3S10p) residues in the nucleus. The increase in H3S10 phosphorylation consequently increased the level of expression of immediate-early gene such as c-fos. This study demonstrated that when breast cancer cells are exposed to alcohol, phosphorylated ROS1 activates MSK1 via ERK1/2 in the MAPK pathway, which then induces modifications to histone residues that regulate gene expression by 14-3-3 protein recruitment, leading to a lack of control of breast cancer cell proliferation.
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影响因子：
4.8
作者：
Hong, Seung-Keun;Yoon, Seunghee;Park, Jong-In
通讯作者：
Park, Jong-In
DOI：
10.1152/ajpgi.00091.2005
发表时间：
2005-12-01
影响因子：
4.5
作者：
Park, PH;Lim, RW;Shukla, SD
通讯作者：
Shukla, SD
DOI：
10.1124/jpet.111.186775
发表时间：
2012-02-01
影响因子：
3.5
作者：
James, Taryn T.;Aroor, Annayya R.;Shukla, Shivendra D.
通讯作者：
Shukla, Shivendra D.
影响因子：
14.9
作者：
Drobic B;Pérez-Cadahía B;Yu J;Kung SK;Davie JR
通讯作者：
Davie JR
DOI：
10.1111/j.1530-0277.2010.01239.x
发表时间：
2010-09-01
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Aroor AR;James TT;Jackson DE;Shukla SD
通讯作者：
Shukla SD