Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse.
Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse.
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在低酶负荷下提高新型纤维素酶混合物的效率，用于碱催化的常压甘油有机溶剂预处理的甘蔗渣的高固体消化。
DOI：
10.1016/j.biortech.2021.125505
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发表时间：
2021-07
影响因子：
11.4
通讯作者：
Jiong Hong
中科院分区：
文献类型：
--
作者：
Marknoah Chinenye Nwamba;Guojie Song;Fubao Sun;Marie Rose Mukasekuru;Hongyan Ren;Qing Zhang;Tishuang Cao;Huaming Wang;Haiyan Sun;Jiong Hong
在生物质糖化过程中以较低的纤维素酶浓度获得高水平的可发酵糖对于确保经济可行且工业相关的水解过程至关重要。因此，本研究使用低纤维素酶负荷（2 mg 蛋白质/g 干燥基质）的新型纤维素酶制剂 (LT4)，评估了通过补料分批饲喂整合辅助酶和添加剂对甘蔗渣高固体水解的可能促进作用。水解从初始 8% 固体负载开始，随后分别在 6 小时、18 小时和 24 小时添加 4% 固体底物，事实证明，结合优化的添加剂和辅助酶，对于 20% 高固体糖化来说是最佳的，72 小时后产生 158 g/L 的总糖和 83% 的葡萄糖产率。获得的结果表明，辅助酶和添加剂的整合提供了一种良性方法，可以最大限度地减少酶负荷和木质纤维素杂聚物高固体糖化的成本，同时还提高酶的水解性能。
The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. The results obtained indicate that the integration of accessory enzymes and additives offers a benignant approach to minimizing the enzyme load and cost of high solids saccharification of lignocellulosic heteropolymers while also boosting enzyme hydrolytic performance.