Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver.
Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver.
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DOI：
10.1111/asj.13672
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发表时间：
2021-01
期刊：
影响因子：
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通讯作者：
Zidi Jin;Long Liu;Cheng Xu;Chunchi Yan;Shuo Li;T. Geng;D. Gong
中科院分区：
文献类型：
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作者：
Zidi Jin;Long Liu;Cheng Xu;Chunchi Yan;Shuo Li;T. Geng;D. Gong
鹅脂肪肝是一种特殊类型的非酒精性脂肪肝，保护免受严重脂肪变性相关的有害影响。我们之前的研究结果表明补体C5的抑制可能与此有关，但其机制尚不清楚。因此，在本研究中，我们首先验证了补体基因（包括C5）在鹅脂肪肝形成过程中的表达模式，然后通过高效液相色谱（HPLC）测定肝脏脂肪含量和脂肪酸组成，然后选择差异代谢物治疗HepG2、鹅和小鼠原代肝细胞，旨在探讨C5和鹅脂肪肝炎症抑制的机制。这些数据证实了补体基因（包括C5）在鹅脂肪肝中的抑制作用。脂肪含量显著高于正常肝脏，主要是油酸和棕榈酸造成的差异。高浓度棕榈酸导致鹅原代肝细胞中C5表达下调，而小鼠原代肝细胞和HepG2细胞中C5表达上调。综上所述，包括高水平棕榈酸在内的脂肪肝相关因子对C5表达的调控可能参与了鹅肝免受重度肝脂肪变性的保护作用。
Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.