Characterization of the antigenic lipopolysaccharide O chain and the capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 13
Characterization of the antigenic lipopolysaccharide O chain and the capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 13
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DOI：
10.1128/iai.72.10.5925-5930.2004
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Vinogradov, E
中科院分区：
文献类型：
--
作者：
MacLean, LL;Perry, MB;Vinogradov, E
胸膜肺炎放线杆菌是猪胸膜肺炎的病原菌，其血清学分型对流行病学研究和同源疫苗细胞制剂的研制具有重要意义。血清学基于荚膜多糖（CPS）和脂多糖（LPS）抗原性O-多糖部分（O-PS）的特定化学结构，并且需要了解这些结构才能在分子水平上了解其血清学特异性。对A.已阐明胸膜肺炎血清型1至12的CPS和O-PS;然而，尚未报道与三种新提出的血清型（血清型13、14和15）相关的结构。本文描述了A.胸膜肺炎血清型13。A.胸膜肺炎血清型13 LPS是由L-鼠李糖、2-乙酰氨基-2-脱氧-D-半乳糖和D-半乳糖残基（1：1：2）组成的支链四糖重复单元的聚合物。通过使用水解，甲基化和高碘酸氧化化学方法，以及应用一维和二维H-1和C-13核磁共振光谱和质谱，确定了O链和CPS的结构。A的CPS。胸膜肺炎血清型13被表征为磷壁酸型聚合物。LPS O抗原与A.胸膜肺炎血清型7。CPS具有1，3-聚（甘油磷酸）磷壁酸I型聚合物的独特结构，并构成定义A的大分子。胸膜肺炎血清型13抗原特异性。
Serotyping of Actinobacillus pleuropneumoniae, the etiologic agent of porcine pleuropneumonia, is important for epidemiological studies and for the development of homologous vaccine cell preparations. The serology is based on the specific chemical structures of capsular pollysaccharides (CPSs) and lipopolysaccharide (LPS) antigenic O-polysaccharide moieties (O-PSs), and knowledge of these structures is required for a molecular-level understanding of their serological specificities. The structures of A. pleuropneumoniae serotype 1 to 12 CPSs and O-PSs have been elucidated; however, the structures associated with three newly proposed serotypes (serotypes 13, 14, and 15) have not been reported. Herein we described the structures of the antigenic O-PS and CPS of A. pleuropneumoniae serotype 13. The O-PS of the A. pleuropneumoniae serotype 13 LPS is a polymer of branched tetrasaccharide repeating units composed Of L-rhamnose, 2-acetamido-2-deoxy-D-galactose, and D-galactose residues (1:1:2). By use of hydrolysis, methylation, and periodate oxidation chemical methods together with the application of one- and two-dimensional H-1 and C-13 nuclear magnetic resonance spectroscopy and mass spectrometry, the structures of the O chain and CPS were determined. The CPS of A. pleuropneumoniae serotype 13 was characterized as a teichoic-acid type polymer. The LPS O antigen was identical to the O-PS produced by A. pleuropneumoniae serotype 7. The CPS has the unique structure of a 1,3-poly(glycerol phosphate) teichoic acid type I polymer and constitutes the macromolecule defining the A. pleuropneumoniae serotype 13 antigenic specificity.