RESPONSE TO ANOXIA IN RICE AND WHEAT SEEDLINGS - CHANGES IN THE PH OF INTRACELLULAR COMPARTMENTS, GLUCOSE-6-PHOSPHATE LEVEL, AND METABOLIC-RATE
RESPONSE TO ANOXIA IN RICE AND WHEAT SEEDLINGS - CHANGES IN THE PH OF INTRACELLULAR COMPARTMENTS, GLUCOSE-6-PHOSPHATE LEVEL, AND METABOLIC-RATE
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DOI：
10.1104/pp.95.3.760
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发表时间：
1991-03-01
期刊：
影响因子：
7.4
通讯作者：
RAGG, E
中科院分区：
文献类型：
--
作者：
MENEGUS, F;CATTARUZZA, L;RAGG, E
P-31 核磁共振波谱用于测量活体组织的细胞内 pH 值。 缺氧导致水稻 (Oryza sativa L. var arborio) 芽的细胞质快速酸化，pH 从 7.4 升至 7.0，水稻是一种高度耐缺氧的物种。 缺氧10分钟后酸化完成。 随后细胞质和液泡均碱化。 在不耐缺氧的小麦芽中，Triticum aestivum L. var MEK，相同的处理引起了更剧烈的细胞质酸化，从pH 7.4到6.6，发生在2小时内。 细胞质酸化随着缺氧的进展而继续，并且没有与在水稻中观察到的液泡碱化相媲美。 在小麦中，戒断也会导致葡萄糖-6-磷酸水平和代谢率降低。 它还导致组织中无机磷酸盐的大量损失。 相反，在水稻中，葡萄糖-6-磷酸盐水平和代谢率增加，并且完全不存在无机磷酸盐从组织中渗漏。 这些结果与植物耐缺氧的机制相关进行讨论。
P-31 nuclear magnetic resonance spectroscopy was used to measure intracellular pH in living tissues. Oxygen deprivation caused fast cytoplasmic acidification from pH 7.4 to 7.0 in shoots of rice, Oryza sativa L. var arborio, a species highly resistant to anoxia. Acidification was complete after 10 minutes of anoxia. Alkalinization of both cytosplasm and vacuole followed thereafter. In the anoxia intolerant wheat shoots, Triticum aestivum L. var MEK, the same treatment caused a sharper cytoplasmic acidification, from pH 7.4 to 6.6, which occurred during a period of 2 hours. Cytoplasmic acidification continued with progress of anoxia and there was no vacuolar alkalinization comparable to the one observed in rice. In wheat oxygen, withdrawal also caused the reduction of both glucose-6-phosphate level and of metabolic rate. It also induced heavy losses of inorganic phosphate from tissues. Conversely, in rice, glucose-6-phosphate level and metabolic rate were increased and inorganic phosphate leakage from tissues was completely absent. These results are discussed in relation to the mechanisms of plant resistance to anoxia.