Effect of Plasma Treatment of Titanium Surface on Biocompatibility
Effect of Plasma Treatment of Titanium Surface on Biocompatibility
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DOI：
10.3390/app9112257
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发表时间：
2019-06-01
影响因子：
2.7
通讯作者：
Okazaki, Joji
中科院分区：
文献类型：
--
作者：
Ujino, Daiga;Nishizaki, Hiroshi;Okazaki, Joji
最近有报道称种植体的骨整合受到表面润湿性的影响。大量的体内研究证实了亲水性和细胞粘附之间的关系。浓碱改善了纯钛的生物相容性。对这种处理增加亲水性的机制进行了研究。在本研究中，我们使用大气压等离子体处理，以提高材料表面的亲水性。目的是评估其对材料与大鼠骨髓的初始粘附以及随后分化为硬组织的影响。超亲水性诱导纯钛表面与piezobrush，一个简单的，紧凑的替代传统的大气压等离子体装置。扫描电子显微镜（SEM）或扫描探针显微镜（SPM）观察未证实结构变化。X射线光电子能谱（XPS）分析表明，氢氧化物的形成和C峰的减少。还观察到接触角减小。经处理的样品具有较高的值，在体外牛血清白蛋白（BSA）吸附，大鼠骨髓（RBM）细胞的初始粘附，碱性磷酸酶活性（ALP）活性测试，骨分化相关的因素比未经处理的对照。本研究表明，通过大气压等离子体处理与piezobrush钛超亲水性的诱导影响RBM细胞粘附和骨分化，而不改变表面特性。
It was recently reported that implant osseointegration is affected by surface wettability. The relationship between hydrophilicity and cell adhesion was corroborated by numerous in vivo studies. Concentrated alkali improves the biocompatibility of pure titanium. Research was conducted on the mechanism by which this treatment increases hydrophilicity. In the present study, we used atmospheric pressure plasma processing to enhance the hydrophilicity of the material surface. The aim was to assess its influences on the initial adhesion of the material to rat bone marrow and subsequent differentiation into hard tissue. Superhydrophilicity was induced on a pure titanium surface with a piezobrush, a simple, compact alternative to the conventional atmospheric pressure plasma device. No structural change was confirmed by Scanning electron microscope (SEM) or scanning probe microscopy (SPM) observation. X-ray photoelectron spectroscopy (XPS) analysis presented with hydroxide formation and a reduction in the C peak. A decrease in contact angle was also observed. The treated samples had higher values for in vitro bovine serum albumin (BSA) adsorption, rat bone marrow (RBM) cell initial adhesion, alkaline phosphatase activity (ALP) activity tests, and factors related to bone differentiation than the untreated control. The present study indicated that the induction of superhydrophilicity in titanium via atmospheric pressure plasma treatment with a piezobrush affects RBM cell adhesion and bone differentiation without altering surface properties.