Increase in the basal transcriptional activity of the human foamy virus internal promoter by the homologous long terminal repeat promoter in cis.
Increase in the basal transcriptional activity of the human foamy virus internal promoter by the homologous long terminal repeat promoter in cis.
复制标题
通过顺式同源长末端重复启动子增加人泡沫病毒内部启动子的基础转录活性。
DOI：
10.1093/nar/21.18.4226
复制
发表时间：
1993
影响因子：
14.9
通讯作者：
R. Flügel
中科院分区：
文献类型：
--
作者：
M. Löchelt;M. Aboud;R. Flügel
人泡沫状或泡沫状逆转录病毒HFV是一种复杂的外源性逆转录病毒，除了三种经典的逆转录病毒基因gag、pol和env外，还编码几种bel基因。最近报道HFV含有两个功能活性启动子，这两个启动子都被HFV反式激活蛋白Bel 1强烈反式激活。第二个内部帽位点的出现强调了HFV基因组的复杂性。我们分析了HFV长末端重复序列启动子与位于bel基因上游env 3 '端的内部启动子之间是否存在干扰。构建了携带两种不同指示基因的重组克隆，一种在U3启动子的控制下，另一种在内部启动子的控制下。在长末端重复启动子存在下，不被Bel 1反式激活的内部启动子的基础转录活性的部分增加了2 - 3倍。在这些构建体中，两种HFV启动子的Bel 1反式激活速率没有改变。
The human foamy or spumaretrovirus HFV is a complex and exogenous retrovirus that encodes several bel genes besides the three classical retroviral genes gag, pol, and env. HFV was recently reported to contain two functionally active promoters that are both strongly trans-activated by the HFV trans-activator protein Bel 1. The occurrence of a second internal cap site underscores the complexity of the HFV genome. We have analysed whether there is interference between the HFV long terminal repeat promoter and the internal promoter located in the 3' end of env upstream of the bel genes. Recombinant clones were constructed that carry two different indicator genes, one under the control of the U3 promoter, the other under the control of the internal promoter. The portion of the basal transcriptional activity of the internal promoter that is not trans-activated by Bel 1 was increased two- to threefold in the presence of the long terminal repeat promoter. The rate of trans-activation by Bel 1 of both HFV promoters was not altered in these constructs.