Metabolome Analysis of Multi-Connected Biparental Chromosome Segment Substitution Line Populations
Metabolome Analysis of Multi-Connected Biparental Chromosome Segment Substitution Line Populations
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多连接双亲染色体片段取代系群体的代谢组分析
DOI：
10.1104/pp.18.00490
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发表时间：
2018-10-01
期刊：
影响因子：
7.4
通讯作者：
Luo, Jie
中科院分区：
文献类型：
--
作者：
Chen, Jie;Wang, Jilin;Luo, Jie
代谢组学分析与先进的遗传群体相结合是研究植物代谢组的强大工具。然而，水稻（Oryza sativa）代谢组的遗传分析主要使用天然种质或单个双亲群体进行。在这里，来自具有共同循环遗传背景的三个相互连接的染色体片段替代系群体的旗叶被用来剖析水稻代谢多样性。我们有效地使用了多个相互关联的双亲群体，通过将 ACC10 (A /Z)、明慧 63 (M/Z) 和日本晴 (N/Z) 的基因组片段引入镇山 97 构建，来绘制代谢数量性状位点 (mQTL)。总共产生了 1,587 个 mQTL，其中分别从 A/Z、M/Z 和 N/Z 染色体片段替换系群体中获得了 684、479 和 722 个，我们为 367 个 mQTL 指定了 99 个候选基因。此外，通过联合连锁分析专门生成了 1,001 个 mQTL，并指定了 25 个候选基因。其中几个候选物已得到验证，例如用于吡哆醇及其衍生物体内含量的 LOC_Os07g01020 和用于顺式玉米素葡萄糖基转移酶活性的 LOC_Os04g25980。我们提出了一种新的 O-甲基芹菜素 C-戊糖苷生物合成途径，并通过精细定位证明 LOC_Os04g11970 编码该途径的一个组成部分。我们假设甲基化芹菜素可能赋予植物抗病性。这项研究证明了使用多个相互关联的群体来产生大量真正的 mQTL 的力量。在功能代谢组学和各自代谢物的指定候选物的可能特征的背景下讨论了综合结果。
Metabolomic analysis coupled with advanced genetic populations represents a powerful tool with which to investigate the plant metabolome. However, genetic analyses of the rice (Oryza sativa) metabolome have been conducted mainly using natural accessions or a single biparental population. Here, the flag leaves from three interconnected chromosome segment substitution line populations with a common recurrent genetic background were used to dissect rice metabolic diversity. We effectively used multiple interconnected biparental populations, constructed by introducing genomic segments into Zhenshan 97 from ACC10 (A /Z), Minghui 63 (M/Z), and Nipponbare (N/Z), to map metabolic quantitative trait loci (mQTL). A total of 1,587 mQTL were generated, of which 684, 479, and 722 were obtained from the A/Z, M/Z, and N/Z chromosome segment substitution line populations, respectively, and we designated 99 candidate genes for 367 mQTL. In addition, 1,001 mQTL were generated specifically from joint linkage analysis with 25 candidate genes assigned. Several of these candidates were validated, such as LOC_Os07g01020 for the in vivo content of pyridoxine and its derivative and LOC_Os04g25980 for cis-zeatin glucosyltransferase activity. We propose a novel biosynthetic pathway for O-methylapigenin C-pentoside and demonstrated that LOC_Os04g11970 encodes a component of this pathway through fine-mapping. We postulate that the methylated apigenin may confer plant disease resistance. This study demonstrates the power of using multiple interconnected populations to generate a large number of veritable mQTL. The combined results are discussed in the context of functional metabolomics and the possible features of assigned candidates underlying respective metabolites.