Oxalate toxicity in LLC-PK1 cells, a line of renal epithelial cells
Oxalate toxicity in LLC-PK1 cells, a line of renal epithelial cells
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DOI：
10.1016/s0022-5347(01)66402-4
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发表时间：
1996-03-01
影响因子：
6.6
通讯作者：
Menon, M
中科院分区：
文献类型：
--
作者：
Scheid, C;Koul, H;Menon, M
目的：本研究评估了高浓度草酸盐对肾上皮细胞毒性的可能性。材料和方法：将LLC-PK 1细胞亚融合培养物暴露于草酸盐，并评估其对细胞形态、细胞膜对活性染料的渗透性、DNA完整性和细胞密度的影响。草酸盐暴露在具有较高浓度（> 140 μ M）的LLC-PK 1细胞的光学显微镜外观中产生时间和浓度依赖性变化。诱导显著的胞质空泡化和核固缩。暴露于草酸盐也增加了细胞膜对活体染料的渗透性，促进了DNA片段化，在高浓度（350 μ M）下，结论：由于高浓度的草酸盐对肾上皮细胞具有毒性，高尿酸可能导致几种形式的肾脏疾病，包括草酸钙结石病和终末期肾脏疾病。
Purpose: The present studies assessed the possibility that high concentrations of oxalate may be toxic to renal epithelial cells.Materials and Methods: Subconfluent cultures of LLC-PK1 cells were exposed to oxalate, and the effects on cell morphology, membrane permeability to vital dyes, DNA integrity and cell density were assessed.Results: Oxalate exposure produced time- and concentration-dependent changes in the light microscopic appearance of LLC-PK1 cells with higher concentrations (> 140 mu M.) inducing marked cytosolic vacuolization and nuclear pyknosis. Exposure to oxalate also increased membrane permeability to vital dyes, promoted DNA fragmentation and, at high concentrations (350 mu M. free oxalate), induced a net loss of LLC-PK1 cells.Conclusions: Since high concentrations of oxalate can be toxic to renal epithelial cells, hyperoxaluria may contribute to several forms of renal disease including both calcium oxalate stone disease and end-stage renal disease.