Intermediate-sized filaments and specific markers in a human salivary gland adenocarcinoma cell line and its nude mouse tumors.
Intermediate-sized filaments and specific markers in a human salivary gland adenocarcinoma cell line and its nude mouse tumors.
复制标题
人唾液腺腺癌细胞系及其裸鼠肿瘤中的中等大小的丝状体和特异性标记物。
作者：
M. Sato;Y. Hayashi;T. Yanagawa;H. Yoshida;Y. Yura;M. Azuma;A. Ueno
本文用免疫过氧化物酶法检测了体外或裸鼠体内增殖的人涎腺腺癌细胞系HSG中三种不同类型的中间纤维（IF）和癌胚抗原、S-100蛋白、分泌成分、乳铁蛋白、肌球蛋白、原肌球蛋白和肌动蛋白等特异性抗原的表达。发现培养的HSG细胞表达三种不同类型的由角蛋白、波形蛋白和结蛋白抗体定义的IF。在34 ℃和37 ℃体外增殖的HSG细胞中，观察到原肌球蛋白和癌胚抗原的表达，但未检测到肌球蛋白和S-100蛋白。肌动蛋白、乳铁蛋白和分泌成分的表达分别限于39 ℃和37 ℃下培养的HSG细胞。将HSG细胞移植到裸鼠体内，建立了具有侵袭和转移等恶性特征的裸鼠系统。在原发性肿瘤中的IFs的表达仅限于角蛋白和结蛋白IFs，而角蛋白，波形蛋白和结蛋白IFs的共表达在区域淋巴结和肺的转移性肿瘤中存在的一些肿瘤细胞中观察到。此外，肌动蛋白，肌球蛋白，原肌球蛋白，和S-100蛋白的表达被发现在转移性肿瘤，而肌球蛋白和S-100蛋白未检测到在原发性肿瘤。此外，尽管在培养的HSG细胞及其原发肿瘤中未发现嗜酸细胞改变，但转移肿瘤几乎被具有嗜酸细胞改变的肿瘤细胞占据。
The adenocarcinoma cell line HSG from human salivary gland, which proliferates in vitro or in nude mice, was examined by the immunoperoxidase method for the expression of three different types of intermediate-sized filaments (IFs) and of specific antigens such as carcinoembryonic antigen, S-100 protein, secretory component, lactoferrin, myosin, tropomyosin, and actin. The cultured HSG cells were found to express three different types of IFs defined by antibodies to keratin, vimentin, and desmin. In HSG cells proliferating in vitro at 34 degrees C and 37 degrees C but not at 39 degrees C, the expression of tropomyosin and carcinoembryonic antigen was observed, although myosin and S-100 protein were not detected. The expressions of actin, lactoferrin, and secretory component were restricted to cultured HSG cells at 39 degrees C and 37 degrees C, respectively. Transplantation of HSG cells into nude mice resulted in the establishment of a nude mouse system with malignant characteristics such as invasion and metastasis. The expression of IFs in the primary tumors was restricted to keratin and desmin IFs, whereas coexpression of keratin, vimentin, and desmin IFs was observed in some neoplastic cells present in the metastatic tumors in regional lymph nodes and lung. In addition, expression of actin, myosin, tropomyosin, and S-100 protein was found in the metastatic tumors, whereas myosin and S-100 protein were not detected in the primary tumors. Moreover, the metastatic tumors were almost occupied by the neoplastic cells with oncocytic changes, although oncocytic change was not found in the cultured HSG cells and their primary tumors.