Metabolism of C-apolipoproteins: kinetics of C-II, C-III1 and C-III2, and VLDL-apolipoprotein B in normal and hyperlipoproteinemic subjects.
Metabolism of C-apolipoproteins: kinetics of C-II, C-III1 and C-III2, and VLDL-apolipoprotein B in normal and hyperlipoproteinemic subjects.
复制标题
C-载脂蛋白的代谢：正常和高脂蛋白血症受试者中 C-II、C-III1 和 C-III2 以及 VLDL-载脂蛋白 B 的动力学。
作者：
M. Huff;N. Fidge;P. Nestel;T. Billington;B. Watson
对15名正常和高脂血症患者注射125i标记的VLDL后，研究了个体C载脂蛋白（C- ii、C- iii1和C- iii2）的转换和代谢。通过分析等电聚焦分离极低密度脂蛋白（VLDL）和高密度脂蛋白（HDL）中的C载脂蛋白，随后对染色带进行密度扫描和放射测定，获得比活性值。在15名受试者中，13名C-II、C-III1和C-III2的动力学最好地描述为单池模型，而2名受试者表现为双指数动力学。VLDL和HDL的比活性-时间曲线是不重叠的，表明高脂血症患者和正常人的C载脂蛋白交换都很迅速。在每个受试者中，C- ii、C- iii1和C- iii2的半衰期相似，这表明每种C载脂蛋白的合成和分解代谢机制相似。可交换性C-II （1.0-5.8 mg/kg）、C-III1 （2.6-20 mg/kg）和C-III2 （2.0-13 mg/kg）的质量随着血浆甘油三酯浓度的增加而增加。C-II的通量值为1.0-2.8 mg/d / kg， C-III1为1.6-5.6 mg/d / kg， C-III2为1.2-3.1 mg/d / kg，但它们与血浆甘油三酯水平无关。然而，不可逆的分数分解代谢率与C载脂蛋白（和甘油三酯）质量呈负相关，这表明C载脂蛋白池大小的扩大与较慢的去除有关，这是由于富含甘油三酯的VLDL颗粒在血浆中停留时间较长。同时进行的VLDL中B载脂蛋白动力学的类似发现证实了这一点。
The turnover and metabolism of the individual C apolipoproteins (C-II, C-III1, and C-III2) were studied following the injection of 125I-labeled VLDL into 15 normal and hyperlipoproteinemic subjects. The C apolipoproteins from very low density lipoprotein (VLDL) and high density lipoprotein (HDL) were separated by analytical isoelectric focusing, and subsequent densitometric scanning and radioassay of the stained bands yielded values for specific activity. In 13 of 15 subjects, kinetics of C-II, C-III1, and C-III2 were best described by a one-pool model, whereas two subjects showed biexponential kinetics. The specific activity-time curves for VLDL and HDL were super-imposable, indicating rapid exchange of all C apolipoproteins in hyperlipidemic as well as in normal subjects. In each subject the half-life was similar for C-II, C-III1, and C-III2, which suggests similar synthesis and catabolic mechanisms for each C apolipoprotein. The mass of exchangeable C-II (range 1.0-5.8 mg/kg), C-III1 (2.6-20 mg/kg), and C-III2 (2.0-13 mg/kg) increased with plasma triglyceride concentrations. Values for flux of C-II were 1.0-2.8 mg/d per kg, for C-III1 1.6-5.6 mg/d per kg, and for C-III2 1.2-3.1 mg/d per kg, but they were not related to levels of plasma triglyceride. However the irreversible fractional catabolic rates were inversely related to C apolipoprotein (and triglyceride) mass, suggesting that expansion in C apolipoprotein pool size is related to slower removal, due to the longer residence time of triglyceride-rich VLDL particles in plasma. This was confirmed by similar findings for B apolipoprotein kinetics in VLDL carried out simultaneously.
DOI：
10.1016/s0021-9258(19)70647-4
发表时间：
1980-09
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Eberhard WindlerS;Yu-sheng Chaos;Richard;Havela
通讯作者：
Eberhard WindlerS;Yu-sheng Chaos;Richard;Havela