TANDEM MASS-SPECTROMETRY OF SMALL, MULTIPLY CHARGED OLIGONUCLEOTIDES
TANDEM MASS-SPECTROMETRY OF SMALL, MULTIPLY CHARGED OLIGONUCLEOTIDES
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DOI：
10.1016/1044-0305(92)85019-g
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发表时间：
1992-01-01
影响因子：
3.2
通讯作者：
GLISH, GL
中科院分区：
文献类型：
--
作者：
MCLUCKEY, SA;VANBERKEL, GJ;GLISH, GL
来自各种小寡核苷酸（n = 4-8）钠盐电喷雾电离的多电荷阴离子已在四极离子阱中进行串联质谱（MS/MS）分析。 即使在通常不利于观察高 MS/MS 效率的条件下，也观察到所有离子都以高效率解离。 总产物离子信号的大部分可归因于单裂解反应，其中母离子电荷在不同组合的两个产物离子之间共享。 在每种情况下，观察到最容易的反应是腺嘌呤阴离子的损失。 然后观察到该反应之后是糖的磷酸二酯键的3'C-O键的裂解，从中失去了腺嘌呤。
Multiply charged anions derived from electrospray ionization of the sodium salts of various small oligonucleotides (n = 4-8) have been subjected to tandem mass spectrometry (MS/MS) in a quadrupole ion trap. All ions were observed to dissociate with high efficiencies even under conditions not ordinarily conducive for the observance of high MS/MS efficiency. Large fractions of the total product ion signal could be attributed to single-cleavage reactions with the parent ion charge shared among the two product ions in various combinations. In every case, the most facile reaction was observed to be the loss of the adenine anion. This reaction was then observed to be followed by cleavage of the 3' C-O bond of phosphodiester linkage of the sugar from which the which the adenine had been lost.