A maize resistance gene functions against bacterial streak disease in rice
A maize resistance gene functions against bacterial streak disease in rice
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DOI：
10.1073/pnas.0503023102
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发表时间：
2005-10
影响因子：
11.1
通讯作者：
Bingyu Zhao;Xing-hua Lin;J. Poland;H. Trick;J. Leach;S. Hulbert
中科院分区：
文献类型：
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作者：
Bingyu Zhao;Xing-hua Lin;J. Poland;H. Trick;J. Leach;S. Hulbert
虽然谷类作物都属于禾本科(禾本科)，但它们的大多数疾病都是特定物种特有的。因此，一种给定的谷类植物通常对其他牧草的病害具有抵抗力，这种非寄主抵抗力通常是稳定的。为了确定在远缘牧草之间转移非寄主抗性基因(R基因)以控制特定病害的可行性，我们克隆了一个识别水稻病原菌Xanthomonas oryzae PV的玉米R基因。引起细菌性条纹病的稻瘟病。条纹病是亚洲水稻的一种重要病害，在水稻中还没有发现简单的遗传抗性来源。虽然X.O.PV.Oryzicola不会在玉米上致病，我们鉴定了一个玉米基因Rxo1，它对不同的病原菌产生抗性反应。令人惊讶的是，Rxo1还控制着对无关病原体Burkholderia andropogonis的抗性，这种病原体会引起高粱和玉米的细菌条纹。因此，同一基因控制着玉米对病原菌和非病原菌的抗性反应。Rxo1具有核苷酸结合位点-富含亮氨酸的重复结构，类似于许多先前发现的R基因。更重要的是，Rxo1基因导入水稻后发挥了转基因的作用，证明了非寄主R基因在谷物间转移的可行性，并为细菌性条斑病的防治提供了有价值的工具。
Although cereal crops all belong to the grass family (Poacea), most of their diseases are specific to a particular species. Thus, a given cereal species is typically resistant to diseases of other grasses, and this nonhost resistance is generally stable. To determine the feasibility of transferring nonhost resistance genes (R genes) between distantly related grasses to control specific diseases, we identified a maize R gene that recognizes a rice pathogen, Xanthomonas oryzae pv. oryzicola, which causes bacterial streak disease. Bacterial streak is an important disease of rice in Asia, and no simply inherited sources of resistance have been identified in rice. Although X. o. pv. oryzicola does not cause disease on maize, we identified a maize gene, Rxo1, that conditions a resistance reaction to a diverse collection of pathogen strains. Surprisingly, Rxo1 also controls resistance to the unrelated pathogen Burkholderia andropogonis, which causes bacterial stripe of sorghum and maize. The same gene thus controls resistance reactions to both pathogens and nonpathogens of maize. Rxo1 has a nucleotide-binding site-leucine-rich repeat structure, similar to many previously identified R genes. Most importantly, Rxo1 functions after transfer as a transgene to rice, demonstrating the feasibility of nonhost R gene transfer between cereals and providing a valuable tool for controlling bacterial streak disease.