Examination of nanoparticle inactivation of Campylobacter jejuni biofilms using infrared and Raman spectroscopies.
Examination of nanoparticle inactivation of Campylobacter jejuni biofilms using infrared and Raman spectroscopies.
复制标题
使用红外和拉曼光谱检查空肠弯曲杆菌生物膜的纳米粒子失活。
DOI：
10.1111/j.1365-2672.2012.05373.x
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发表时间：
2012
影响因子：
4
通讯作者：
Konkel,ME
中科院分区：
文献类型：
--
作者：
Lu,X;Weakley,AT;Aston,DE;Rasco,BA;Wang,S;Konkel,ME
目的探讨氧化锌纳米颗粒对空肠弯曲菌生物膜的灭活作用及其机制。方法与结果采用L−1的0、0·6、1·2和6 mm ol的氧化锌纳米颗粒对空肠弯曲菌生物膜、空肠弯曲菌和生物膜进行了抑菌试验。红外光谱和扫描电子显微镜表明，氧化锌纳米颗粒穿透胞外聚合物(EPS)而不破坏EPS，并直接与固着细菌细胞相互作用。拉曼光谱显示，用纳米氧化锌处理空肠后，苯二酚的结构发生了变化，核酸也发生了损伤。DNA损伤的机制很可能是由于活性氧物种(ROS)的产生。基于光谱的偏最小二乘回归模型可以从傅立叶变换红外光谱(FT-IR)中预测细菌生物膜内存活的固着细胞数(≥log4cfu，估计均方根误差&0.36)。结论纳米氧化锌颗粒具有抗菌活性。氧化锌纳米颗粒在1h内穿透生物膜EPS，对EPS无损伤，并与生物膜中的固着细胞直接相互作用。由于ROS的产生，DNA/RNA碱基的改变似乎导致了空肠细胞的死亡。研究氧化锌纳米粒子的意义和影响可能为消除环境中的空肠生物膜提供一种现实的策略。
AimsTo investigate inactivation effect and mechanism of zinc oxide nanoparticles (ZnO NPs) activity againstCampylobacter jejunibiofilms.Methods and ResultsZnO NPs with concentrations of 0, 0·6, 1·2 and 6 mmol l−1were employed in antimicrobial tests againstCamp. jejuniplanktonic cells and biofilms.Campylobacter jejunisessile cells in biofilms were more resistant to a low concentration of ZnO NPs when compared to planktonic cells. The ZnO NPs penetrated the extracellular polymeric substance (EPS) without damage to the EPS and directly interacted with the sessile bacterial cells, as determined using infrared spectroscopy and scanning electron microscopy. Raman spectroscopy shows alterations in quinone structures and damage to nucleic acids followingCamp. jejunitreatment with ZnO NPs. The mechanism of DNA damage is most likely due to the generation of reactive oxygen species (ROS). Spectroscopic‐based partial least squares regression (PLSR) models could predict the number of surviving sessile cell numbers within a bacterial biofilm (≥log 4 CFU, root mean square error of estimation <0·36) from Fourier transform infrared (FT‐IR) spectral measurements.ConclusionsZnO NPs were found to have antimicrobial activity againstCamp. jejunibiofilms. ZnO NPs penetrated the biofilm EPS within 1 h without damaging it and interacted directly with sessile cells in biofilms. Alterations in the DNA/RNA bases, which are owing to the generation of ROS, appear to result inCamp. jejunicell death.Significance and Impact of the StudyZnO NPs may offer a realistic strategy to eliminateCamp. jejunibiofilms in the environment.