In vivo behaviors of highly flexible paper consisting of ultralong hydroxyapatite nanowires
In vivo behaviors of highly flexible paper consisting of ultralong hydroxyapatite nanowires
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DOI：
10.1002/jbm.b.34819
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发表时间：
2021-03-04
影响因子：
3.4
通讯作者：
Zhu, Ying-Jie
中科院分区：
文献类型：
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作者：
Kashiwada, Hajime;Shimizu, Yoshinaka;Zhu, Ying-Jie
本研究旨在探讨一种由超长羟基磷灰石纳米线组成的薄而柔韧的羟基磷灰石（HAP）纸的生物相容性。在Wistar大鼠的颅骨上制备直径为8.8mm的圆形骨缺损，以暴露硬脑膜。将类似大小的圆形HAP纸放置在骨缺损的底部。分别于术后2、4、8周处死大鼠，对实验切片进行显微CT扫描和组织学观察。覆盖有纤维组织的HAP纸显示没有炎性细胞浸润，并且它们的厚度随着时间的推移而减小。抗酒石酸酸性磷酸酶阳性破骨细胞样细胞被诱导周围的HAP纸的边缘沿着与HAP纸的剥落。在骨缺损区域观察到新形成的骨，无论是与HAP纸直接接触还是通过薄的纤维组织。由于在HAP纸表面检测到碱性磷酸酶活性，因此证实了HAP纸诱导的成骨细胞分化。这些结果表明，HAP纸可以诱导成骨而不会引起任何有害影响。高度柔性的HAP纸有助于骨再生治疗的进一步发展。
This study was conducted in order to investigate biological compatibility of a thin and flexible hydroxyapatite (HAP) paper which consists of ultralong hydroxyapatite nanowires. Circular-shaped cranial bone defects with a diameter of 8.8 mm were prepared to expose the dura maters in Wistar rats. The similar-sized, circular-shaped HAP paper was placed at the bottom of the bone defects. After 2, 4, and 8 weeks, the rats were sacrificed, and the experimental sections were examined by micro-CT scanning and histological observation. The HAP paper covered with fibrous tissues showed no inflammatory cell infiltration, and their thicknesses decreased over time. Tartrate-resistant acid phosphatase-positive osteoclast-like cells were induced around the edges of the HAP paper along with the exfoliation of the HAP paper. The newly-formed bones were observed in the bone-defected areas, either with a direct contact with the HAP paper or through thin fibrous tissues. The HAP paper-induced osteoblast differentiation was confirmed since the alkaline phosphatase activities were detected on the surfaces of the HAP paper. These results indicated that the HAP paper may induce osteogenesis without causing any harmful effects. The highly flexible HAP paper can contribute to further development of bone regenerative therapy.