Amino acid metabolism reprogramming in response to changing growth environment in Ginkgo biloba leaves
Amino acid metabolism reprogramming in response to changing growth environment in Ginkgo biloba leaves
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DOI：
10.1016/j.lwt.2021.111276
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发表时间：
2021-03-26
影响因子：
6
通讯作者：
Wang, Guibin
中科院分区：
文献类型：
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作者：
Guo, Ying;Wang, Mengke;Wang, Guibin
氨基酸广泛存在于银杏叶中，在树体生长和银杏产品质量检测中起着重要作用。然而，氨基酸的生物合成和积累还没有得到充分的研究，特别是对复杂环境条件的响应。在此，我们整合和分析了生长在三个不同生境的银杏叶片的转录组和代谢组，包括不同性别(雌雄)的克隆同质材料，以揭示氨基酸代谢的环境响应机制。主成分分析揭示了银杏叶片氨基酸积累的环境依赖性，即不同环境的银杏叶片氨基酸含量差异显著，而生长在同一环境中的雌雄银杏叶片氨基酸含量差异不大。加权基因相关网络分析表明，受多种转录因子调控的苯丙烷类生物合成途径结构基因和受体样激酶编码基因的过度表达与氨基酸的积累密切相关。此外，我们还指出，环境条件直接影响C、N流在叶片中的分配，从而影响银杏茶的产量和品质(碳水化合物和氨基酸含量)。
Widely distributed in Ginkgo leaves, the amino acids play an important role in the tree growth and Ginkgo products quality determination. Nevertheless, the biosynthesis and accumulation of amino acids have not been fully studied, especially in response to complex environmental conditions. Here, we integrated and analyzed transcriptome and metabolome from Ginkgo leaves originated from clonal homogeneous materials including their different genders (female and male) grown in three different habitats to reveal the environmental response mechanism of amino acid metabolism. Principal component analysis revealed the environmental dependence of amino acid accumulation in Ginkgo leaves, that is, the content of amino acids differed significantly between samples from different environments, while it was similar between female and male samples grown in the same environment. Weighted gene correlation network analysis suggested that the overexpression of structural genes in phenylpropanoid biosynthesis pathway and genes encoding receptor-like kinases, which was regulated by multiple transcription factors, were closely related to the amino acids' accumulation. Further, we indicated that environmental conditions directly affected the C and N flow allocation in leaves, thereby having an impact on the yield and quality (carbohydrates and amino acids contents) of Ginkgo tea.