Effects of intermittent high glucose on oxidative stress in endothelial cells
Effects of intermittent high glucose on oxidative stress in endothelial cells
复制标题
间歇性高糖对内皮细胞氧化应激的影响
DOI：
10.1007/s00592-009-0140-5
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发表时间：
2010-12-01
影响因子：
3.8
通讯作者：
Su, Qing
中科院分区：
文献类型：
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作者：
Ge, Qin-Min;Dong, Yan;Su, Qing
本研究旨在探讨间歇性高糖诱导猪髂骨内皮细胞(PIECs)氧化应激的机制。间歇性或持续性高糖培养3d或6d，流式细胞仪检测细胞内活性氧(ROS)含量，测定细胞内平均荧光强度。用荧光素化学发光法测定NADPH氧化酶活性。间歇性高糖可诱导PIECs产生更多的ROS，而NADPH氧化酶活性在恒定和间歇性高糖条件下均有增加，且以间歇性高糖作用更为明显(P<；P<；0.05)。综上所述，间歇性高糖诱导的PIECs比持续高糖诱导更多的ROS，这一效应似乎至少部分与NADPH氧化酶的激活有关。血糖波动可能参与血管并发症的发生。
The objective of this study is to explore the mechanism of oxidative stress induced by intermittent high glucose in porcine iliac endothelial cells (PIECs). The PIECs were exposed to intermittent or constant high glucose for 3 or 6 days, and the mean fluorescent intensity (MFI) was measured via intracellular reactive oxygen species (ROS) captured by flow cytometry. The NADPH oxidase activity was measured by chemiluminescence with lucigenin. Intermittent high glucose induced a greater over-production of ROS than constant high glucose in PIECs; the NADPH oxidase activity was increased under both constant and intermittent high glucose conditions, being more marked in the latter (P< 0.05). In conclusion, intermittent high glucose induced more ROS in PIECs than constant high glucose, this effect seemed to be, at least in part related to the enhanced activation of NADPH oxidase. Glucose fluctuation may be involved in the development of vascular complications.