Global analysis of stress-regulated mRNA turnover by using cDNA arrays
Global analysis of stress-regulated mRNA turnover by using cDNA arrays
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DOI：
10.1073/pnas.162212399
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发表时间：
2002-08-06
影响因子：
11.1
通讯作者：
Gorospe, M
中科院分区：
文献类型：
--
作者：
Fan, JS;Yang, XL;Gorospe, M
cDNA阵列技术已被证明是监测基因表达模式全球变化的有力手段。在这里，我们提出了一种方法，扩展了目前的实用程序的cDNA阵列，允许评估的相对作用的转录和mRNA营业额在全球范围内的基因表达，与目前的个别基因的基因分析。这种方法，它涉及到大规模的杂交模式产生的稳态mRNA与新转录（核运行）RNA的比较，被用来证明在调节基因表达的mRNA周转几种条件下的压力的重要性。
cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.