RAPID CDNA SEQUENCING (EXPRESSED SEQUENCE TAGS) FROM A DIRECTIONALLY CLONED HUMAN INFANT BRAIN CDNA LIBRARY
RAPID CDNA SEQUENCING (EXPRESSED SEQUENCE TAGS) FROM A DIRECTIONALLY CLONED HUMAN INFANT BRAIN CDNA LIBRARY
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DOI：
10.1038/ng0893-373
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发表时间：
1993-08-01
期刊：
影响因子：
30.8
通讯作者：
VENTER, JC
中科院分区：
文献类型：
--
作者：
ADAMS, MD;SOARES, MB;VENTER, JC
通过对 1,600 多个克隆进行部分测序来评估专门用于生成表达序列标签 (EST) 的人类婴儿大脑 cDNA 文库。该文库专为 EST 测序而构建，其优点包括使用定向克隆、大小选择、线粒体和核糖体转录物数量极少、polyA 尾短、非重组体少以及转录物代表性广泛。根据与公共数据库中 320 多个不同基因的匹配，识别出了 37% 的克隆。其中，鉴定出两种与阿尔茨海默病淀粉样前体蛋白相似的蛋白质。
A human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs) was evaluated by partial sequencing of over 1,600 clones. Advantages of this library, constructed for EST sequencing, include the use of directional cloning, size selection, very low numbers of mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants and a broad representation of transcripts. 37% of the clones were identified, based on matches to over 320 different genes in the public databases. Of these, two proteins similar to the Alzheimer's disease amyloid precursor protein were identified.