FASCIN, AN ECHINOID ACTIN-BUNDLING PROTEIN, IS A HOMOLOG OF THE DROSOPHILA SINGED GENE-PRODUCT
FASCIN, AN ECHINOID ACTIN-BUNDLING PROTEIN, IS A HOMOLOG OF THE DROSOPHILA SINGED GENE-PRODUCT
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DOI：
10.1073/pnas.90.19.9115
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发表时间：
1993-10-01
影响因子：
11.1
通讯作者：
WULFKUHLE, J
中科院分区：
文献类型：
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作者：
BRYAN, J;EDWARDS, R;WULFKUHLE, J
一个肌成束蛋白的cDNA，在棘皮动物肌动蛋白捆绑蛋白，已被克隆，测序和表达。蛋白质的预测质量几乎等于55 kDa，类似于从海胆卵中纯化的肌成束蛋白所观察到的。细菌表达的肌成束蛋白与针对海胆卵肌成束蛋白制备的抗体反应。Fascin与果蝇中的signed基因（sn）产物具有很强的序列相似性，并且与55 kDa的人类肌动蛋白捆绑蛋白具有相似性。没有发现广泛的相似性与其他已知的肌动蛋白结合/捆绑蛋白，表明这是一个独立的基因家族。
A cDNA for fascin, an actin-bundling protein in echinoderms, has been cloned, sequenced, and expressed. The predicted mass of the protein is almost-equal-to 55 kDa, similar to that observed for fascin purified from sea urchin eggs. Bacterially expressed fascin reacts with antibodies prepared against sea urchin egg fascin. Fascin has a strong sequence similarity to the singed gene (sn) product in Drosophila and has similarities with a 55-kDa human actin-bundling protein. No extensive similarities were found with other known actin-binding/bundling proteins, indicating that this is a separate gene family.