Isolation of Extracellular Vesicles from Stem Cells.
Isolation of Extracellular Vesicles from Stem Cells.
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DOI：
10.1007/978-1-4939-7253-1_32
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Chen Z;Li Y;Yu H;Shen Y;Ju C;Ma G;Liu Y;Kim IM;Weintraub NL;Tang Y
移植的诱导多能干细胞（IPSC）和胚胎干细胞（ESC）在缺血组织中表现出增强的存活，并通过旁分泌效应促进邻近细胞的存活。最近的研究表明，干细胞可以分泌细胞外囊泡（EV），它可以在细胞之间穿梭非编码RNA，促进供体干细胞和受体组织之间的细胞间信号传导和通讯。在小鼠急性心肌缺血-再灌注（MI/R）模型中，直接移植IPSC衍生的EV（IPSC-EV）在促进心肌细胞存活和防止心肌细胞凋亡方面非常有效。在这里，我们描述了一个可行的协议，从培养的IPSC纯化EV。
Transplanted induced pluripotent stem cells (IPSC) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC-derived EV (IPSC-EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia–reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC.