ABSENCE OF INTERMEDIATE FILAMENTS IN A HUMAN ADRENAL-CORTEX CARCINOMA-DERIVED CELL-LINE
ABSENCE OF INTERMEDIATE FILAMENTS IN A HUMAN ADRENAL-CORTEX CARCINOMA-DERIVED CELL-LINE
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DOI：
10.1016/0014-4827(86)90081-9
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发表时间：
1986-04-01
影响因子：
3.7
通讯作者：
CHEN, LB
中科院分区：
文献类型：
--
作者：
HEDBERG, KK;CHEN, LB
描述了人肾上腺皮质癌衍生细胞系(SW13)的亚克隆，其通过免疫荧光缺乏已知的五类中间丝(IF)蛋白中的任何一种的表达。通过二维凝胶分析和免疫印迹进一步研究了这些亚克隆中的波形蛋白和角蛋白，结果与免疫荧光结果一致。尽管明显缺乏间质干细胞，但SW13亚克隆已经组织了肌动蛋白和微管细胞骨架网络，保持上皮形态和集落模式，并在培养中生长良好。虽然一种类似的大鼠肝癌细胞系似乎已经停止表达[1]，但这是人类细胞系的第一次这样的报告。虽然罕见，但这些案例提供了证据，证明IF一般对培养的生长不是必不可少的，也不是含有角蛋白的IF特别地对培养的上皮细胞所特有的“鹅卵石”形态或集落状生长模式负责。
Subclones of a human adrenal cortex carcinoma-derived cell line (SW 13) are described which by immunofluorescence lack detectable expression of any of the five known classes of intermediate filament (IF) proteins. Further investigation for vimentin and keratins in these subclones by two-dimensional gel analysis and by immunoblotting gave results consistent with the immunofluorescence results. Despite the apparent absence of IFs, SW13 subclones have organized actin and microtubule cytoskeletal networks, maintain an epithelial shape and colony pattern, and grow well in culture. Although a rat hepatoma cell line which similarly appears to have ceased IF expression has been reported [1], this is the first such report of a human cell line. Although rare, these cases provide evidence that IFs in general are not essential to growth in culture, nor are the keratin-containing IFs in particular necessarily responsible for the ''cobblestone'' morphology or colony-type growth pattern characteristic of cultured epithelial cells.