The use of the o-phthalaldehyde reaction as a sensitive assay for protein and to determine protein in bacterial cells and dental plaque.
The use of the o-phthalaldehyde reaction as a sensitive assay for protein and to determine protein in bacterial cells and dental plaque.
复制标题
使用邻苯二甲醛反应作为蛋白质的灵敏测定并确定细菌细胞和牙菌斑中的蛋白质。
DOI：
10.1016/0003-2697(78)90500-6
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发表时间：
1978
影响因子：
2.9
通讯作者：
W. Bowen
中科院分区：
文献类型：
--
作者：
S. A. Robrish;C. Kemp;W. Bowen
碱性水解蛋白质，然后与邻苯二甲醛反应，允许测定小于10 ng的蛋白质荧光。当用邻苯二甲醛分析完整蛋白质时，反应的灵敏度较低。该反应已应用于牙菌斑和细菌细胞蛋白质含量的分析。
Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protein content of dental plaque and bacterial cells.