Expression of the high mobility group proteins HMGI(Y) correlates with malignant progression in Barrett's metaplasia.
Expression of the high mobility group proteins HMGI(Y) correlates with malignant progression in Barrett's metaplasia.
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高迁移率族蛋白 HMGI(Y) 的表达与 Barrett 化生的恶性进展相关。
DOI：
10.1158/1055-9965.epi-03-0151
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Younes,Mamoun
中科院分区：
文献类型：
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作者：
Chen,Xueyun;Lechago,Juan;Ertan,Atilla;Ergun,Gulchin;Verm,Ray;Bridges,Margaret;Johnson,Craig;Woods,Karen;Meriano,Frank;Chirala,Minni;Younes,Mamoun
高迁移率族蛋白HMGI（Y）的表达已被证明是甲状腺和胰腺病变中恶性肿瘤的标志物，并且与结肠中的恶性进展显著相关。本研究的目的是确定HMGI（Y）表达是否与Barrett化生（BM）的恶性进展相关。对42例BM患者的福尔马林固定石蜡包埋内镜食管活检切片进行HMGI（Y）免疫过氧化物酶染色。其中包括19例异型增生（ND）阴性活检，16例低度异型增生（LGD）/不确定异型增生（IND），7例高度异型增生（HGD）/腺癌（CA）。记录阳性细胞的百分比，并且认为>10%的细胞中的核HMGI（Y）免疫反应性为阳性。使用Fisher精确检验进行统计分析。19例ND中2例（11%）、16例LGD/IND中5例（30%）、7例HGD/CA中7例（100%）HMGI（Y）染色阳性。HGD/CA活检组织HMGI（Y）阳性率显著高于ND（P< 0.0001）或LGD/IND（P= 0.0046）。我们得出结论，HMGI（Y）表达与BM恶性进展显著相关。需要进一步的研究来确定ND或LGD/IND和HMGI（Y）阳性的BM活检是否更有可能进展为腺癌。
Expression of the high mobility group proteins HMGI(Y) has been shown to be a marker of malignancy in thyroid and pancreatic lesions and to correlate significantly with malignant progression in the colon. The aim of this study was to determine whether HMGI(Y) expression is associated with malignant progression in Barrett’s metaplasia (BM). Immunoperoxidase staining for HMGI(Y) was performed on sections of formalin-fixed paraffin-embedded endoscopic esophageal biopsies from 42 patients with BM. These consisted of 19 biopsies negative for dysplasia (ND), 16 with low-grade dysplasia (LGD)/indeterminate for dysplasia (IND), and 7 with high-grade dysplasia (HGD)/adenocarcinoma (CA). The percentage of positive cells was recorded, and nuclear HMGI(Y) immunoreactivity in >10% of the cells was considered positive. Statistical analysis was performed using Fisher’s exact test. Positive HMGI(Y) staining was detected in 2 of 19 (11%) cases ND, 5 of 16 (30%) LGD/IND cases, and 7 of 7 (100%) HGD/CA cases. Biopsies with HGD/CA were significantly more likely to be positive for HMGI(Y) than biopsies ND (P< 0.0001) or with LGD/IND (P= 0.0046). We conclude that HMGI(Y) expression is significantly associated with malignant progression in BM. Additional studies are needed to determine whether BM biopsies that are ND or LGD/IND and positive for HMGI(Y) are more likely to progress to adenocarcinoma.