Proteomic and microRNA Transcriptome Analysis revealed the microRNA-SmyD1 network regulation in Skeletal Muscle Fibers performance of Chinese perch.
Proteomic and microRNA Transcriptome Analysis revealed the microRNA-SmyD1 network regulation in Skeletal Muscle Fibers performance of Chinese perch.
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蛋白质组学和 microRNA 转录组分析揭示了 microRNA-SmyD1 网络对中华鲈鱼骨骼肌纤维性能的调控
DOI：
10.1038/s41598-017-16718-2
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发表时间：
2017-11-28
影响因子：
4.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chu W;Zhang F;Song R;Li Y;Wu P;Chen L;Cheng J;Du S;Zhang J
鱼类肌节由解剖学上分离的快肌纤维和慢肌纤维组成，它们具有不同的代谢和收缩特性。虽然在mRNA水平上研究了快肌和慢肌纤维中的表达谱特性，但在蛋白质组学和microRNA转录组学水平上的综合分析是有限的。本研究首次系统地比较了中华鲟（Siniferica chuatsi）慢肌和快肌的蛋白质组和microRNA转录组。在肌肉组织中共鉴定出2102个蛋白质。其中，99个蛋白质差异上调，400个蛋白质下调，在快肌与慢肌相比。miRNA微阵列显示，在两种类型的肌纤维中鉴定出199个miRNA。与快肌相比，慢肌中有32个miRNAs表达上调，27个下调。具体而言，miR-103和miR-144的表达分别与快肌和慢肌中SmyD 1a和SmyD 1b的表达呈负相关。荧光素酶报告基因检测进一步证实了miR-103和miR-144通过靶向SmyD 1a和SmyD 1b的3′-UTR直接调控SmyD 1a和SmyD 1b的表达。构建的miRNA-SmyD 1相互作用网络可能在控制鲈鱼不同肌纤维类型的发育和性能方面发挥重要作用。
Fish myotomes are comprised of anatomically segregated fast and slow muscle fibers that possess different metabolic and contractile properties. Although the expression profile properties in fast and slow muscle fibers had been investigated at the mRNA levels, a comprehensive analysis at proteomic and microRNA transcriptomic levels is limited. In the present study, we first systematically compared the proteomic and microRNA transcriptome of the slow and fast muscles of Chinese perch (Siniperca chuatsi). Total of 2102 proteins were identified in muscle tissues. Among them, 99 proteins were differentially up-regulated and 400 were down-regulated in the fast muscle compared with slow muscle. MiRNA microarrays revealed that 199 miRNAs identified in the two types of muscle fibers. Compared with the fast muscle, the 32 miRNAs was up-regulated and 27 down-regulated in the slow muscle. Specifically, expression of miR-103 and miR-144 was negatively correlated with SmyD1a and SmyD1b expression in fast and slow muscles, respectively. The luciferase reporter assay further verified that the miR-103 and miR-144 directly regulated the SmyD1a and SmyD1b expression by targeting their 3′-UTR. The constructed miRNA-SmyD1 interaction network might play an important role in controlling the development and performance of different muscle fiber types in Chinese perch.
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影响因子：
10.5
作者：
Chen, PY;Manninga, H;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
4
作者：
Just, Steffen;Meder, Benjamin;Rottbauer, Wolfgang
通讯作者：
Rottbauer, Wolfgang
影响因子：
2.7
作者：
Liu, YW;Shen, TS;Schneider, MF
通讯作者：
Schneider, MF
影响因子：
3.3
作者：
Huang, C. W.;Li, Y. H.;Wu, J. L.
通讯作者：
Wu, J. L.
影响因子：
30.8
作者：
Gottlieb, PD;Pierce, SA;Srivastava, D
通讯作者：
Srivastava, D