Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco
Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco
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DOI：
10.1023/a:1025003628446
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发表时间：
2003-09
影响因子：
2.7
通讯作者：
Qiuli Li;Xiao-rong Gao;Xiaohong Yu;Xingzhi Wang;L. An
中科院分区：
文献类型：
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作者：
Qiuli Li;Xiao-rong Gao;Xiaohong Yu;Xingzhi Wang;L. An
从辽东盐生植物碱蓬中克隆了甜菜碱醛脱氢酶(BADH)基因，并进行了序列测定，并在烟草(Nictiana Tabacum89)中表达。该基因全长1506个碱基对，编码502个氨基酸的多肽。将编码该成熟酶的基因片段克隆到载体pCAMBIA-1301中，并在烟草中表达。Southern blotting分析表明Badh1基因已整合到烟草基因组中。表达BADH的烟草在含200 mM氯化钠的MS培养基上存活，而未转化的植株在20d左右变黄并死亡。
cDNA encoding betaine aldehyde dehydrogenase (BADH) from the halophyteSuaeda liaotungensishas been cloned, sequenced and expressed in tobacco (Nictiana tabacum89). The full-length cDNA is 1506 base pairs (bp) long and encodes a 502 amino-acid polypeptide. The cDNA fragment coding for the mature enzyme was cloned into vector pCAMBIA-1301 for expression in tobacco. Southern blotting analysis showed that that theBadhgene was integrated into the genome of tobacco. Tobacco expressing BADH survived on MS medium containing 200 mM NaCl, whereas the untransformed plants turned yellow after about 20 d and died.