Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism
Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism
复制标题
DOI：
10.1111/j.1524-475x.2007.00228.x
复制
发表时间：
2007-05-01
影响因子：
2.9
通讯作者：
Mathur, Anshu B.
中科院分区：
文献类型：
--
作者：
Cheema, Sangeeta K.;Chen, Ellen;Mathur, Anshu B.
RNA干扰和短干扰RNA（siRNA）已被证明可有效降低靶基因的表达，并为促进和指导功能组织的生长以用于修复和重建组织工程应用提供了有价值的工具。siRNA是一种基因沉默机制，涉及双链RNA介导的序列特异性mRNA降解，是控制细胞行为的有力机制。使用siRNA减少靶基因的表达可以诱导一种或多种组织诱导因子的表达，指导干细胞或祖细胞的分化，或去除抑制再生的因子，这可以用于组织形成的基础研究或用于促进体内再生的应用。通过血管生成、骨和神经再生以及伤口愈合领域内的具体实例来说明siRNA的潜力。此外，解决了有效递送siRNA用于组织工程应用的挑战。siRNA代表了研究和/或促进组织形成的有力工具，并且存在许多机会用于鉴定促进组织再生的靶标和开发有效的递送系统。
RNA intereference and short-interfering RNA (siRNA) have been proven to be effective at decreasing the expression of target genes and provide a valuable tool for promoting and directing the growth of functional tissues for repair and reconstructive tissue engineering applications. siRNA is a gene-silencing mechanism that involves double-stranded RNA-mediated sequence-specific mRNA degradation and is a powerful mechanism for controlling cell behavior. The use of siRNA to reduce the expression of a target gene can induce the expression of one or more tissue-inductive factors, direct the differentiation of stem or progenitor cells, or remove a factor that inhibits regeneration, which can be useful in fundamental studies of tissue formation or in applications to promote in vivo regeneration. The potential of siRNA is illustrated through specific examples within the fields of angiogenesis, bone and nerve regeneration, and wound healing. In addition, challenges to deliver siRNA effectively for tissue engineering applications are addressed. siRNA represents a powerful tool to investigate and/or promote tissue formation, and numerous opportunities exist for identifying targets that promote regeneration of tissue and developing effective delivery systems.