RNA sequencing analysis identifies the metabolic and developmental genes regulated by BbSNF1 during conidiation of the entomopathogenic fungus Beauveria bassiana
RNA sequencing analysis identifies the metabolic and developmental genes regulated by BbSNF1 during conidiation of the entomopathogenic fungus Beauveria bassiana
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DOI：
10.1007/s00294-014-0462-x
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发表时间：
2015-05-01
期刊：
影响因子：
2.5
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
He, Pu-Hong;Wang, Xiu-Xiu;Ying, Sheng-Hua
条件作用促进真菌在环境中的扩散和生存，是球孢白僵菌生物防治潜力的决定因素。SNF1/AMPK蛋白激酶是真菌发育和能量代谢的重要调节因子，在丝状真菌的分生过程中起着至关重要的作用。在先前的研究中，已经确定球孢白僵菌同源物（BbSNF1）控制分生孢子的产生。本研究表明，Delta BbSNF1突变体菌丝和分生孢子发育延迟，但分生孢子形态发生无明显变化。消融BbSNF1显著改变了分生过程中细胞内氨基酸代谢稳态，导致精氨酸、丙氨酸、缬氨酸、苯丙氨酸、赖氨酸、亮氨酸和谷氨酸7种氨基酸含量显著降低。上述氨基酸均能不同程度地恢复突变体的分生率（43.3% ~ 300%）。转录组学分析揭示了许多可能受BbSNF1调控并与分生孢子发育相关的靶基因，这些基因主要参与代谢、细胞拯救和运输。特别是，与氨基酸降解相关的4个类别在上调基因中被过度代表，与氨基酸生物合成相关的3个类别在下调基因中被过度代表。此外，Delta BbSNF1突变体的分生过程上游和中央调控基因，以及其他调控基因和细胞骨架基因的表达水平均有所降低。我们的数据表明，SNF1激酶通过调节代谢和中央调控来促进球孢白僵菌的分生。
Conidiation promotes fungal dispersal and survival in the environment, and is a determinant for the biocontrol potential of Beauveria bassiana. The SNF1/AMPK protein kinases function as an important regulator of fungal development and energy metabolism, and play a crucial role in conidiation of the filamentous fungi. In previous study, it has been established that the B. bassiana homolog (BbSNF1) controls conidial production. This study showed that the Delta BbSNF1 mutants displayed a delayed development of mycelia and conidia, but the conidiophore morphogenesis was not significantly changed in the mutants. Ablation of BbSNF1 significantly changed the metabolic homeostasis of intracellular amino acids during conidiation, and caused a notable reduction in the contents of seven amino acids (i.e., arginine, alanine, valine, phenylalanine, lysine, leucine, and glutamic acid). All above amino acids could recover conidiation of the mutants in different extents (ranging from 43.3 to 300 %). Transcriptomic analysis revealed many putative target genes regulated by BbSNF1 and associated with conidial development, and these genes were primarily involved in metabolism, cell rescue, and transport. Particularly, four categories related to the amino acid degradation were over-represented in the up-regulated genes, and three categories related to the amino acid biosynthesis were over-represented in the down-regulated genes. Moreover, the Delta BbSNF1 mutants displayed reduced expression level of the upstream and central regulators of conidiation, as well as the other regulator and cytoskeleton genes. Our data indicate that SNF1 kinase contributes to B. bassiana conidiation by regulating the metabolism and the central regulators of conidiation.