Effects of Interfacial Properties of a Surface Modified Surface Plasmon Resonance Chip on Protein Immobilization Performance
Effects of Interfacial Properties of a Surface Modified Surface Plasmon Resonance Chip on Protein Immobilization Performance
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表面修饰表面等离子体共振芯片界面性质对蛋白质固定化性能的影响
DOI：
10.2116/analsci.33.481
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发表时间：
2017-04-01
影响因子：
1.6
通讯作者：
Zeng, Qiang
中科院分区：
文献类型：
--
作者：
Wu, Juan;Jiang, Yanbin;Zeng, Qiang
为了验证表面等离子体共振（SPR）芯片的界面性质与其对固定化抗IgG的SPR响应之间的相关性，采用巯基十一烷酸、聚乙二醇二丙烯酸酯（PEG）、PEG基共聚物和环糊精偶联的PEG，通过自组装或自由基聚合方法对SPR芯片进行了修饰。用原子力显微镜、椭圆偏振扫描仪和接触角仪对膜厚和亲水性等界面性质进行了表征。修饰芯片上的人IgG的固定化通过经由酰胺键的EDC/NHS活化来实现。SPR反应的变化反映了固定IgG和游离抗IgG之间的结合，并通过Langmuir等温线评估了结合能力。如所观察到的，IgG和抗IgG之间的吸附受到不同改性剂的界面性质的影响，使得具有更薄和更亲水层的芯片可能导致更高的SPR响应，产生更大的蛋白质相互作用的吸附平衡常数。
In order to confirm the correlation between interfacial properties of modified surface plasmon resonance (SPR) chips and their SPR responses to immobilized anti-IgG, SPR chips were modified by mercaptoundecanoic acid, poly(ethylene glycol) diacrylate (PEG), PEG-based copolymer and cyclodextrin coupled PEG using self-assembled or radical polymerization methods. The resulting interfacial properties such as film thickness and hydrophilicity were characterized by AFM, elliptic polarization scanners and contact angle meter. Immobilization of human IgG on the modified chips was achieved by EDC/NHS activation through an amide bond. The association between fixed IgG and free anti-IgG was reflected by the variation of SPR responses and the binding ability was evaluated by Langmuir isotherms. As observed, the adsorption between IgG and anti-IgG was affected by the interfacial properties of different modifiers, such that a chip with a thinner and more hydrophilic layer may result in a higher SPR response, producing a larger adsorption equilibrium constant for protein interaction.