Genetic analysis of craniofacial traits in the medaka
Genetic analysis of craniofacial traits in the medaka
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DOI：
10.1534/genetics.106.068460
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发表时间：
2007-12-01
期刊：
影响因子：
3.3
通讯作者：
Shinya, Minori
中科院分区：
文献类型：
--
作者：
Kimura, Tetsuaki;Shimada, Atsuko;Shinya, Minori
家庭和双胞胎表明，显着的遗传成分强调了颅面形态的个体差异。在当前的研究中，我们对来自两个近交青鳉 (Oryzias latipes) 品系 HNI 和 Hd-rR 的 100 个人的 444 个颅面特征进行了量化。在青鳉头部区域的数字图像中测量定义的标志之间的相对距离。两个品系之间共有379个性状存在显着差异，表明许多颅面性状是由遗传因素控制的。其中，89 个性状通过 HNI 和 Hd-rR 杂交的 184 个 F-2 后代的区间作图进行了分析。我们确定了 66 个颅面特征的数量性状基因座。连锁群 (LG) 9 和 11 的赔率得分的最高对数为 6.2。性状 L33，对应于眼睛水平处头部长度与头部高度的比率，映射到 LG9；性状 V15，对应于在眼睛后面测量的鼻子长度与头部宽度的比率，映射到 LG11。我们的初步结果证实了青鳉作为复杂性状（例如颅面形态）遗传分析模型系统的潜力。
Family and twin suggest that a substantial genetic component underlines individual differences in craniofacial morphology. In the current study, we quantified 444 craniofacial traits in 100 individuals from two inbred medaka (Oryzias latipes) strains, HNI and Hd-rR. Relative distances between defined landmarks were measured in digital images of the medaka head region. A total of 379 traits differed significantly between the two strains, indicating that many craniofacial traits are controlled by genetic facrors. Of these, 89 traits were analyzed via interval mapping of 184 F-2 progeny from an intercross between HNI and Hd-rR. We identified quantitative trait loci for 66 craniofacial traits. The highest logarithm of the odds score was 6.2 for linkage group (LG) 9 and 11. Trait L33, which corresponds to the ratio of head length to head height at eye level, mapped to LG9; trait V15, which corresponds to the ratio of snout length to head width measured behind the eyes, mapped to LG11. Our initial results confirm the potential of the medaka as a model system for the genetic analysis of complex traits such as craniofacial morphology.