Surfing and Swimming of Ejaculated Sperm in the Mouse Oviduct
Surfing and Swimming of Ejaculated Sperm in the Mouse Oviduct
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DOI：
10.1095/biolreprod.115.135418
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发表时间：
2016-04-01
影响因子：
3.6
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
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作者：
Ishikawa, Yu;Usui, Tomoyuki;Baba, Tadashi
为了在输卵管壶腹部完成受精，射出的精子需要通过女性生殖道迁移。然而，这一基本过程在很大程度上仍然未知。在本研究中，我们重点研究了输卵管平滑肌收缩在精子迁移中的作用。给小鼠注射普非溴铵，Padrin，有效地抑制了肌肉输卵管的收缩，导致受精率以剂量依赖的方式下降，并阻止了输卵管液体在峡部周围的高速来回/穿梭流动。无论Padrin给药与否，在壶腹附近都没有发现穿梭流动。在峡部，精子形成一个紧密的集合，与穿梭流动同步。在壶腹附近，精子聚集逐渐松散，然后完全消失。注射Padrin时，峡部未见精子聚集现象。这些结果表明，肌性输卵管收缩在峡部精子集合体的形成以及精子集合体向输卵管中段的运输中起着重要作用。精子的运动性对于精子从输卵管中段向壶腹的迁移是必不可少的。
To accomplish fertilization in the oviductal ampulla, ejaculated sperm are required to migrate through the female reproductive tract. However, this fundamental process largely remains unknown. In this study, we focused on the role of oviductal smooth muscle (myosalpinx) contractions in the sperm migration. Administration of prifinium bromide, padrin, to mice effectively suppressed myosalpinx contractions, resulting in a decreased rate of fertilization in a dose-dependent manner, and an abrogation of high-speed back-and-forth/shuttling flows of oviductal fluids around the isthmus. Regardless of padrin administration, no shuttling flows were found near the ampulla. In the isthmus, sperm formed a tight assemblage that was synchronized with the shuttling flows. The sperm assemblage was gradually loosened and then completely abolished near the ampulla. No sperm assemblage was formed in the isthmus when padrin was administrated. These results suggest that myosalpinx contractions play important roles in the formation of sperm assemblage in the isthmus, and in the transport of the assemblage to the middle region of the oviduct. It is also suggested that the motility of sperm is essential for the migration of sperm from the middle oviductal region to the ampulla.