Listeria monocytogenes: cultivation and laboratory maintenance.
Listeria monocytogenes: cultivation and laboratory maintenance.
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DOI：
10.1002/9780471729259.mc09b02s31
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发表时间：
2013-11-05
影响因子：
--
通讯作者：
D'Orazio, Sarah E F
中科院分区：
文献类型：
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作者：
Jones, Grant S;D'Orazio, Sarah E F
本单元描述了实验室培养和储存革兰氏阳性兼性单核细胞增生李斯特菌的一般程序。基本方案适用于广泛的应用，包括微生物遗传学，以及体外和体内感染研究。还讨论了常用的单核增生乳杆菌菌株、血清型和生长参数。
This unit describes general procedures for the lab cultivation and storage of the Gram-positive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics, and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed.