Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region
Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region
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DOI：
10.1079/ber2002177
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发表时间：
2002-08-01
影响因子：
1.9
通讯作者：
Komazaki, S
中科院分区：
文献类型：
--
作者：
Toda, S;Komazaki, S
蓟马是世界范围内广泛农作物的重要害虫，如水果、蔬菜和观赏作物。已知11种蓟马是日本的水果害虫，并且几种物种经常在同一宿主植物上共同出现（Umeya et al.，1988; Tsuchiya等人，1992年）。蓟马是一种非常小的昆虫，在外观上表现出高度的相似性，特别是在未成熟阶段，这使得它们在物种水平上非常难以识别。在针对具体物种的防治方案中，及早准确地识别蓟马物种非常重要，特别是对于已经获得杀虫剂抗性的蓟马（Roehrdanz，1997年）。例如，在日本，花蓟马（Frankliniella occurientalis）（Pergande）和棕榈蓟马（Thrips帕尔米Karny）（Thysanoptera：Thripidae）对商业上使用的一些杀虫剂的敏感性降低（Hayashi，1992; Morishita，1993; Tatara & Suzuki，1993）。根据形态特征，宫崎& Kudo（1986）发表了日本13种蓟马二龄幼虫检索表。然而，这种歧视需要相当多的分类学专业知识和时间，和第一龄幼虫是无法区分的。Murai（1990）报道了一种同工酶分析方法，可以区分9个物种的未成熟阶段。自F.在这项研究（Hayase & Fukuda，1991年）之后，occupientalis入侵了日本，没有关于这个物种的可比数据。酶电泳的主要缺点是酶
Thrips are important pests of a wide range of agricultural crops worldwide, such as fruit, vegetables and ornamental crops. Eleven species of thrips are known to be pests of fruit in Japan and several species often co-occur on the same host plant (Umeya et al., 1988; Tsuchiya et al., 1992). Thrips are very small insects that show a high degree of similarity in appearance, particularly during the immature stages, which can make them extremely difficult to identify at the species level. Early and accurate identification of thrips species is important in species-specific control programmes, especially for thrips that have acquired insecticide resistance (Roehrdanz, 1997). For example, Frankliniella occidentalis (Pergande) and Thrips palmi Karny (Thysanoptera: Thripidae) have reduced susceptibility to some insecticides used commercially in Japan (Hayashi, 1992; Morishita, 1993; Tatara & Suzuki, 1993).Reliable identification of the immature stages is advantageous when applying early control measures. Based on morphological characteristics, Miyazaki & Kudo (1986) published a key to second instar larvae of 13 species of thrips occurring in Japan. However, such discrimination requires considerable taxonomic expertise and time, and first instar larvae were not distinguishable. Murai (1990) reported an isozyme analysis method that discriminated between the immature stages of nine species. Since F. occidentalis invaded Japan after this study (Hayase & Fukuda, 1991), no comparable data for this species are available. The main drawback with enzyme electrophoresis is that enzyme