Analysis of genetic relationships among strains of groups A, C and G streptococci by random amplified polymorphic DNA analysis
Analysis of genetic relationships among strains of groups A, C and G streptococci by random amplified polymorphic DNA analysis
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DOI：
10.1099/00222615-45-4-278
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发表时间：
1996-10-01
影响因子：
3
通讯作者：
LambertZechovsky, N
中科院分区：
文献类型：
--
作者：
Bert, F;Picard, B;LambertZechovsky, N
本文应用随机扩增多态性DNA（RAPD）技术对A、C、G群链球菌进行了种间和种内鉴别。(GGS)、7个S. dysgalactiae、11 S. equisimilis、4个S. canis，3个S. equi和8个S.小动物。3个单引物的组合数据可区分出82个类型，RAPD分析结果与以往基于DNA-DNA同源性和MLEE的物种分类结果基本一致。多个引物的平行使用显著提高了该技术的种内分型效率，RAPD分析对GAS和GGS的区分能力高于MLEE，但两种技术的区分结果并不完全一致，RAPD可区分电泳类型相同的菌株，而MLEE可区分PCR类型相同的菌株，RAPD分析也没有区分所有GAS菌株与不同的生物型，其已经很高的判别力进一步提高了伴随生物分型。
Random amplified polymorphic DNA (RAPD) analysis was evaluated for its capacity to distinguish species and strains within species of groups A, C and G streptococci, The 99 strains tested, previously typed by multilocus enzyme electrophoresis (MLEE), included 41 group A streptococci (Streptococcus pyogenes), 25 group G Streptococcus spp. (GGS), seven S. dysgalactiae, 11 S. equisimilis, four S. canis, three S. equi and eight S. zooepidemicus. The combined data obtained with three single primers distinguished 82 types, RAPD analysis provided taxonomic results that were in general agreement with previous species classification based on DNA-DNA homology and MLEE. The intraspecies typing efficiency of the technique was significantly improved by the parallel use of several primers, RAPD analysis had greater discriminatory power than MLEE for GAS and GGS, There was not total agreement between the two techniques as RAPD distinguished strains with identical electrophoretic types, whereas MLEE differentiated strains with identical PCR types, RAPD analysis did nor distinguish all GAS strains with different biotypes and its already high discriminatory power was further enhanced by concomitant biotyping.