Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye
Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye
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DOI：
10.1016/j.toxicon.2009.07.008
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发表时间：
2010-01-01
期刊：
影响因子：
2.8
通讯作者：
Li, Pengcheng
中科院分区：
文献类型：
--
作者：
Feng, Jinhua;Yu, Huahua;Li, Pengcheng
霞水母是一种广泛分布于中国沿海地区的水母，因其蜇人能力和由此产生的公共卫生危害而引起关注。我们使用一种新的技术从水母囊中提取水母毒液，本研究调查了C。野崎毒结果表明，该囊内容物对草鱼有极强的毒性，产生典型的神经毒素毒性。ID 50约为0.6 μ g蛋白质/g鱼。毒素样品在-80 ℃下保持稳定，但48小时后，在-20 ℃下致死率下降80%。在65-80 ℃和pH 3.5的范围内观察到毒液的稳定性差。毒液被蛋白水解酶胰蛋白酶水解。毒液的分级分离产生两个蛋白质条带，分子量为60 kDa和50 kDa。我们的结果首次证明了C.野崎会产生致命的毒素。这些特点突出了需要新的活性毒素的分离和分子特征。(C)2009爱思唯尔有限公司保留所有权利。
Cyanea nozakii Kishinouye, a jellyfish widely distributed in coastal areas of China, has garnered attention because of its stinging capacity and the resulting public health hazard. We used a recently developed technique to extract jellyfish venom from nematocysts; the present study investigates the lethality of C. nozakii venom. The nematocyst contents were extremely toxic to the grass carp, Ctenopharyngodon idellus, producing typical neurotoxin toxicity. The ID50 was about 0.6 mu g protein/g fish. Toxin samples were stable when kept at -80 degrees C, but after 48 h, an 80% decline in lethality occurred at -20 degrees C. Poor stability of the venom was observed within the range of 65-80 degrees C and at pH 3.5. The venom was hydrolyzed by a proteolytic enzyme, trypsin. Fractionation of the venom yielded two protein bands with molecular weights of 60 kDa and 50 kDa. Our results provide the first evidence that C. nozakii produces lethal toxins. These characteristics highlight the need for the isolation and molecular characterization of new active toxins in C nozakii. (C) 2009 Elsevier Ltd. All rights reserved.