Imaging of cancer invasion and metastasis using green fluorescent protein
Imaging of cancer invasion and metastasis using green fluorescent protein
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DOI：
10.1016/s0959-8049(00)00155-6
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发表时间：
2000-08-01
影响因子：
8.4
通讯作者：
Segall, JE
中科院分区：
文献类型：
--
作者：
Condeelis, JS;Wyckoff, J;Segall, JE
使用绿色荧光蛋白对肿瘤细胞进行荧光标记，使研究人员能够打开转移的“黑匣子”，以便可视化肿瘤细胞在活组织中的行为。对离开原发性肿瘤的细胞的分析表明，高转移性细胞能够更有效地向血管极化，而转移性差的细胞在与血液相互作用时更频繁地碎裂。此外，似乎有更多的宿主免疫系统细胞与转移性肿瘤相互作用。在靶器官如肺或肝中停滞后，大多数肿瘤细胞进入休眠或休眠状态。一小部分停滞的细胞形成转移。在某些靶器官中，肿瘤细胞的迁移可增强形成转移的能力。(C)2000爱思唯尔科技有限公司版权所有。
The use of green fluorescent protein to fluorescently tag tumour cells has allowed investigators to open the "black box" of metastasis in order to visualise the behaviour of tumour cells in living tissues. Analysis of cells leaving the primary tumour indicates that highly metastatic cells are able to polarise more effectively towards blood vessels while poorly metastatic cells fragment more often when interacting with blood. In addition, there appear to be greater numbers of host immune system cells interacting with metastatic tumours. After arresting in target organs such as the lungs or liver, most tumour cells become dormant or apoptose. A small fraction of the arrested cells form metastases. In some target organs, migration of tumour cells may enhance the ability to form metastases. (C) 2000 Elsevier Science Ltd. All rights reserved.